| 常徽1 王湛1 袁丽佳1 付钰洁1,2 糜漫天1.桑葚花色苷提取物对乳腺癌细胞凋亡及线粒体膜电位的影响[J].,2012,12(22):4236-4240 |
| 桑葚花色苷提取物对乳腺癌细胞凋亡及线粒体膜电位的影响 |
| Effect of Anthocyanin Extract of Mulberry Fruit on the Apoptosis andthe Mitochondrial Membrane Potential of Breast Cancer Cells |
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| DOI： |
| 中文关键词: 桑葚花色苷 乳腺癌细胞 凋亡 线粒体膜电位 |
| 英文关键词: Mulberry fruit anthocyanin extract Breast cancer Apoptosis Mitochondrial membrane potential |
| 基金项目:国家自然科学基金（30901194）；第三军医大学青年创新基金（2009XQN14） |
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| 中文摘要: |
| 目的：观察桑葚花色苷提取物对人乳腺癌细胞株MDA-MB-453、MDA-MB-231 和MCF-7 细胞凋亡及线粒体膜电位的影响。
方法：利用超声辅助乙醇萃取法提取桑葚花色苷，pH 示差法测定提取物花色苷总含量，以50、100 和150 mg/mL 桑葚花色苷提取
物作用三种乳腺癌细胞MDA-MB-231、MDA-MB-453 和MCF-7 24 h，采用Annexin V/PI 双染流式细胞分析法检测细胞凋亡水平
变化，JC-1 探针染色激光共聚焦扫描显微镜观察MDA-MB-453 细胞线粒体膜电位水平变化。结果：凋亡分析结果表明，桑葚花色
苷提取物作用后三种乳腺癌细胞凋亡率均升高，显示出促凋亡效应，且具有剂量- 效应关系，100 和150 mg/mL 组凋亡率显著升
高（P<0.05）。激光共聚焦扫描显微镜检测结果显示，桑葚花色苷提取物作用24 h，可使MDA-MB-453 细胞线粒体膜电位显著下
降，表现为红色/ 绿色荧光的比值显著降低（P<0.05）。结论：桑葚花色苷提取物可显著降低乳腺癌细胞线粒体膜电位，并促发细胞
凋亡。 |
| 英文摘要: |
| Objective: To study the effect of anthocyanin extract of mulberry fruit on the apoptosis and the mitochondrial
membrane potential of breast cancer cells MDA-MB-453, MDA-MB-231 and MCF-7. Methods: Preparation of anthocyanin-rich extract
frommulberryfruitswas carried out by ultrasonic extraction with acidified-ethanol. The breast cancer cells MDA-MB-453, MDA-MB-231
and MCF-7 were reated with 50, 100 or 150 mg/mL of the anthocyanin-rich extract for 24h; the cells apoptosis were analyzed by
Annexin V/PI dyeing and flow cytometric assay. To characterize the upstream factors involved in the intrinsic apoptosis pathway, the
mitochondrial permeability of MDA-MB-453 cells were measured by JC-1 staining and the laser confocal scanning microscopy. Results:
The apoptosis analysis results indicated that the anthocyanin-rich mulberry fruit extract treated for 24 h, the apoptosis rate of these three
breast cells in 100 and 150 mg/mL treated groups markedly increased (P<0.05), exhibiting a dose-effect relationship. The observation by
laser confocal scanning microscopy suggested that the anthocyanin-rich extract significantly decreased the mitochondrial membrane
potential of breast cancer MDA-MB-453 cell; the ratio of JC-1 red/green fluorescence was significantly decreased (P<0.05). Conclusion:
The anthocyanin-rich extract significantly decreased the mitochondrial membrane potential of breast cancer cells and induced apoptosis. |
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