| 王菲吕楠张奇昕苏靖.新型聚乙烯亚胺衍生物在Hep G2 细胞中转染和毒性的研究[J].,2012,12(22):4205-4207 |
| 新型聚乙烯亚胺衍生物在Hep G2 细胞中转染和毒性的研究 |
| Transfection and Cytotoxicity Studies of Novel PolyethyleneimineDerivative in Hep G2 cells |
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| DOI： |
| 中文关键词: 聚乙烯亚胺衍生物 Hep G2 细胞 转染 细胞毒性 |
| 英文关键词: Polyethyleneimine Hep G2 cells Transfection Cytotoxicity |
| 基金项目:国家自然科学基金项目（81001416） |
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| 中文摘要: |
| 目的：研究以对苯二甲醛（Terephthalaldehyde）为连接剂的聚乙烯亚胺（Polyethyleneimine, PEI）衍生物PEI-Tp 对肝癌细胞
Hep G2 的转染活性和细胞毒性的影响。方法：以荧光素酶质粒作为报告基因，研究高分子和DNA 的复合物在Hep G2 细胞中的
转染活性，用MTT 的方法研究高分子对Hep G2 细胞的毒性。结果：Hep G2 细胞转染结果显示构建的聚乙烯亚胺衍生物PEI-Tp
具有高效输送质粒的能力；细胞毒性结果显示PEI-Tp 随着浓度的增加，其毒性显著低于PEI25 kDa。结论：Hep G2 细胞实验数据
显示PEI-Tp 是一种高效、低毒，在基因治疗领域有相当前景的非病毒载体。 |
| 英文摘要: |
| Objective: To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine (PEI) derivative linking
with Terephthalaldehyde. Methods: Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in Hep G2
cells. Cytotoxicity was detected by MTT method. Results: Hep G2 cells transfection and cytotoxicity results suggested that the gene
carrier (PEI-Tp) expressed equal transfection efficiency with PEI 25kDa and Lipofectamine 2000, and had less cytotoxicity than that of
PEI 25kDa. Conclusion: PEI-Tp could be a highly efficient and less toxic gene carrier which was promising in gene therapy. |
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