| 于文俊1 杨文华2△ 杨向东2 史哲新2 王兴丽2 郝征1 张佳1.PESV 对K562 细胞BCR/ABL 融合基因及凋亡调控因子Bcl-2、Bad
表达的影响[J].,2012,12(21):4015-4018 |
| PESV 对K562 细胞BCR/ABL 融合基因及凋亡调控因子Bcl-2、Bad
表达的影响 |
| The Effects of PESV on the Expression of BCR / ABL Fusion Gene andBcl-2, Bad of Apoptosis Regulators on the K562 Cells |
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| DOI： |
| 中文关键词: PESV K562 细胞系 凋亡 BCR/ABL Bcl-2 Bad |
| 英文关键词: PESV K562 cell lines Apoptosis BCR / ABL Bcl-2 Bad |
| 基金项目:The Effects of PESV on the Expression of BCR / ABL Fusion Gene and
Bcl-2, Bad of Apoptosis Regulators on the K562 Cells |
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| 中文摘要: |
| 目的：探讨PESV 对K562 细胞BCR/ABL 融合基因及凋亡调控因子bcl-2 和bad 表达的影响。方法：将体外培养K562 细
胞，经PESV 处理不同时间后，流式细胞术检测细胞凋亡率，荧光定量RT-PCR 检测BCR/ABL、Bcl-2、Bad mRNA 水平变化。结
果：与对照组相比，PESV 处理后K562 细胞,凋亡率增加, BCR/ABL 融合基因表达降低，抗凋亡相关基因Bcl-2 mRNA 表达降低,
促凋亡基因Bad mRNA 表达增加。结论：PESV 能降低降低K562 细胞BCR/ABL 融合基因的表达，可能通过调节Bcl-2 和Bad 表
达，抑制K562 细胞增殖,促进其凋亡。 |
| 英文摘要: |
| Objective: To investigate the PESV of K562 cells BCR / ABL fusion gene and apoptosis regulators bcl-2 and bad expression.
Methods: K562 cells were cultured in vitro, by PESV for different times, the apoptosis rate by flow cytometry, fluorescence
quantitative RT-PCR detection of BCR / ABL, Bcl-2, Bad mRNA level changes. Results: Compared with the control group, PESV treated
K562 cells, apoptosis increased, BCR / ABL fusion gene reduced expression, anti-apoptotic gene Bcl-2 mRNA expression decreased,
pro-apoptotic gene Bad mRNA expression. Conclusion: PESV reduced in K562 cells can reduce the BCR / ABL fusion gene, may regulate
the expression of Bcl-2 and Bad, inhibit proliferation of K562 cells and promote their apoptosis. |
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