| 卜倩倩1 黄利华2 李明波3 伍勇1.粪肠球菌生物被膜形成早期相关基因的表达研究[J].,2012,12(20):3822-3826 |
| 粪肠球菌生物被膜形成早期相关基因的表达研究 |
| Expression of Genes for Early Biofilm Formation in Enterococcus Faecalis |
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| DOI： |
| 中文关键词: 粪肠球菌 生物被膜 逆转录PCR 实时荧光定量PCR 相关基因 |
| 英文关键词: Enterococcus faecalis Biofilm RT-PCR Real-Time PCR Related genes |
| 基金项目: |
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| 中文摘要: |
| 目的：以粪肠球菌为研究对象，探讨粪肠球菌基因srtA（转肽酶A 编码基因）、esp（肠球菌表面蛋白）与粪肠球菌生物被膜形
成早期的相关性。方法：用逆转录PCR 与实时荧光定量PCR 方法对生物被膜和浮游菌组细菌srtA、esp 两种与生物被膜形成早期
相关的基因其表达进行检测，并进行统计学分析。结果：srtA、esp 基因与粪肠球生物被膜菌早期形成密切相关。生物被膜菌组
srtA、esp 表达量分别是浮游菌组的7.9 与13.5 倍。结论：srtA、esp 基因与粪肠球生物被膜菌形成早期密切相关，可能是生物被膜
早期形成的上调因子。 |
| 英文摘要: |
| Objective: To study the relationship between the expression of srtA and esp and early biofilm formation in Enterococcus
faecalis. Methods: RT-PCR and real-time quantitative PCR method for biofilm and planktonic Enterococcus faecalis groups of srtA
and esp, two kinds of early biofilm formation genes whose expression were detected and analyzed statistically. Results: The srtA and esp
are colsely related to the early biofilm formation in Enterococcus faecalis. The expression quantity of srtA and esp in biofilm group is 7.9
times and 13.5 times respectively of planktonic group. Conclusions: The srtA and esp are colsely related to the biofilm formation, which
promote the early biofilm formation in Enterococcus faecalis probably. |
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