| 初超侯利民△ 刘明辉.深低温冷冻技术的研究进展[J].,2012,12(19):3766-3769 |
| 深低温冷冻技术的研究进展 |
| Research Progress of Profound Hypothermia |
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| DOI： |
| 中文关键词: 玻璃化 深低温 冷冻保护剂 |
| 英文关键词: Vitrification Profound hypothermia Cryoprotectant |
| 基金项目: |
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| 中文摘要: |
| 细胞及组织的深低温保存有较高的临床应用和研究价值，在冷冻保存技术中两个关键领域首先发展的是冷冻控制率。冷冻
保护剂能增加溶液粘性，提高冷冻速率从而保护细胞及组织免受冷冻损伤。对最佳冷冻保护剂的研究为保存临床应用的组织工
程产品提供了理论依据。而玻璃化冷冻近年来越来越受到人们的关注，玻璃化冷冻技术具有冷冻速度快、冻融损伤小，操作简单
等优点，能够提高复苏后的存活率。本文对深低温保护剂的组成、分类、应用及冷冻保存的重大进展和障碍进行了综述。 |
| 英文摘要: |
| Deep cryopreservation of cells and tissues has high clinical and research value. Controlled-rate freezing, one of the
two key fields in cryopreservation techniques, is the first one to be developed. Cryoprotectants protect cells and tissues from freezing
injury by increasing the solution viscosity and the freezing rate. Researches on the optimal Cryoprotectant provide the theory basis for
preservation and clinical application of tissue-engineered products. Vitrification in recent years is getting more and more attention.
Vitrification has the merits such as frozen fast, less freeze-thaw damage and easy operation. It can improve the survival rate after
cryopreservation. In this review, the composition, classification, application and development of Cryoprotectant are demonstrated and the
major strides and obstacles in cryopreservation will be reviewed. |
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