| 王世缘王艳秋△.基于进化足迹法结合位置打分函数的转录因子结合位点预测[J].,2012,12(19):3725-3727 |
| 基于进化足迹法结合位置打分函数的转录因子结合位点预测 |
| 基于进化足迹法结合位置打分函数的转录因子结合位点预测 |
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| DOI： |
| 中文关键词: 进化足迹法 位置打分函数 特异性 |
| 英文关键词: Phylogenetic footprinting Position scoring function Specificity |
| 基金项目:黑龙江省卫生厅项目(2010-213) |
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| 中文摘要: |
| 目的：预测转录因子结合位点。方法：本文从ABS 数据库上下载了人类和啮齿类动物的直系同源的启动子序列，首先使用位
置打分函数对这些启动子序列进行打分，找到候选的转录因子结合位点，并进一步利用进化足迹法对这些候选的转录因子结合
位点进行筛选，只有结合位点同时出现在人类和啮齿类动物的启动子序列才认为是真正的结合位点。结果：在对人类和啮齿类动
物进行转录因子结合位点预测时，与单独使用打分函数的结果相比，进化足迹法与打分函数结合的方法有效的提高了预测结果
的性能，大幅度提供所得预测结果的特异性。结论：进化足迹法结合位置打分矩阵的方法能较为准确有效的预测转录因子结合位
点。 |
| 英文摘要: |
| Objective: Reliable prediction of transcription factor binding sites can help to identify the target genes of transcription
factors and infer the relationship between the binding sites. But the performance of some algorithms has been unreliable with respect to
poor specificity, more efficient algorithms are required. Methods: In this paper, the human and rodent orthologous promoters sequences
are download from the ABS database, position scoring function is used to predict the transcription factor binding sites, phylogenetic
footprinting is to construct alignment of the orthologous promoter sequences, and only the binding site appeared in both the promoters of
human and rodent are selected. Phylogenetic footprinting are used to reduce the number of predicted binding sites. Results: The
predictive results show that compared with position scoring function, the prediction result are improved highly, and the specificity is also
improved significantly. Conclusion: The algorithm presented in this study could be provided some help for the prediction of transcription
factor binding sites. |
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