| 黄敬敬1 魏良洲2 战淑慧1 董开芯1 王传芳1 苏艳华1 董全江1△.幽门螺杆菌毒力基因型与胃癌的相关性研究[J].,2012,12(18):3525-3528 |
| 幽门螺杆菌毒力基因型与胃癌的相关性研究 |
| The Correlation between Virulence Genotypes of HelicobacterPylori and Gastric Cancer |
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| DOI： |
| 中文关键词: 幽门螺杆菌 cagA vacA iceA1 毒力 |
| 英文关键词: Helicobacter pylori cagA vacA iceA1 Virulence |
| 基金项目: |
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| 中文摘要: |
| 目的：研究中国东部地区幽门螺杆菌（H. pylori）cagA、vacA 和iceA1 毒力基因型的分布状况及其与胃癌的相关性。方法：从
52 例病人胃黏膜活检组织（31 例慢性浅表性胃炎，21 例胃癌）中分离培养H. pylori，用PCR 方法扩增分离菌株的cagA、iceA1、
vacAs, i, m 区毒力基因片段，统计并分析上述毒力因素与胃癌发生的相关性。结果：在52 例菌株中，cagA、vacAs1/i1/m1、
vacAs1/i1/m2 和iceA1 的阳性率分别是92.3%（48/52），48.1%（25/52），48.1%（25/52），90.4%（47/52）；所有的胃癌分离株中均为
cagA（+）vacAs1/i1（+）型，vacAm1、vacAm2 和iceA1 在胃癌组中的阳性率分别是47.6%（10/21），52.4%（11/21），95.2%（20/21），与
胃炎组相比，上述基因型的阳性率差异均无显著统计学意义（P>0.05）。结论：cagA、vacAs1/i1/m1、vacAs1/i1/m2 和iceA1 是中国东
部地区H. pylori 的优势基因型；cagA、vacAs1、vacAi1 和iceA1 毒力基因型的存在与胃癌的发生无相关性。 |
| 英文摘要: |
| Objective: To investigate the prevalence of virulent genotypes of Helicobacter pylori (H. pylori) and the relationship
between virulence factors and gastric cancer. Methods: H. pylori were cultured from gastric biopsies obtained from 31 patients with
chronic gastritis and 21 with gastric cancer. The virulence genes, cagA, iceA1, vacA s, i and m regions were amplified by using PCR and
the relationship between virulence factors and gastric cancer was analyzed. Results: The prevalence of cagA, vacAs1/i1/m1, vacAs1/
i1/m2 and iceA1 in 52 isolates were 92.3% (48/52), 48.1% (25/52), 48.1% (25/52), 90.4% (47/52), respectively. All the gastric cancer
isolates were cagA (+) vacAs1/i1 (+), and the positive rates of vacAm1, vacAm2 and iceA1 in group of gastric cancer were 47.6% (10/21),
52.4%(11/21), 95.2%(20/21), respectively. There were no statistically significant differences in the positive rates of all the genotypes
above compared with the positive rates in gastritis isolates (P>0.05). Conclusions: The majority of H. pylori isolated from East China
possess virulent genotypes are cagA, vacAs1/i1/m1, vacAs1/i1/m2 and iceA1 positive. It appeared that the virulence factors of H. pylori
had no significant association with gastric cancer. |
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