| 任东青1 黄晓峰2△ 尹文2△ 韩英3 王瑞安4 李增山4 王爽2 朱疆依3
李永强2 亢君君2.Gilbert 综合征肝活检的超微结构特征*[J].,2012,12(18):3439-3441 |
| Gilbert 综合征肝活检的超微结构特征* |
| Ultrastructural Characteristics in Liver Biopsy of Gilbert's Syndrome* |
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| DOI： |
| 中文关键词: Gilbert 综合征 超微结构 肝细胞 透射电镜 |
| 英文关键词: Gilbert's syndrome Ultrastructure Hepatocyte Transimission electron microscope |
| 基金项目:陕西省自然科学基础研究计划（2009JM4006）；陕西省社发公关项目（2010K15-03-07） |
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| 中文摘要: |
| 目的：确定Gilbert 综合征患者肝组织的超微结构特征，为Gilbert 综合征的诊断和鉴别诊断提供新的方法。方法：按电镜常
规进行标本制备，应用透射电镜对20 例Gilbert 综合征患者肝穿刺活检组织进行超微结构观察。结果：肝细胞可出现巨大线粒体，
常含有副晶格样包涵体、较明显的基质致密颗粒。肝细胞常见脂褐素颗粒增多，多分布于毛细胆管周围肝细胞内。可出现较有特
征性的色素颗粒，大小不等，卵圆形或不规则形，含有电子致密块状颗粒，与电子密度略低的聚集物以及脂滴互相混杂。这些溶酶
体颗粒的基质由细小的、弱嗜锇性的颗粒组成。少数颗粒类似Dubin-Johnson 综合征的颗粒。但颗粒较小，缺少致密核芯结构。结
论：特征性的含粗大电子致密物的溶酶体对Gilbert 综合征的诊断有重要参考价值。 |
| 英文摘要: |
| Objective: To investigate the ultrastructural characteristics in liver biopsy of Gilbert's syndrome and to provide a new
method for diagnosis and differential diagnosis of Gilbert's syndrome. Methods: The liver biopsy specimens were obtained from 20 patients
with Gilbert's syndrome and regularly processed for transmission electron microscopy. Results: Giant mitochrondria often accompanied
with paracrystalline inclusions was presented, and numerous matrix dense granules could be found in hepatocytes. An increase in
lipofusin granules in hepatocytes was a common finding and appeared in the periphery of bile canaliculus. The specific pigment granules
with variable size, oval or irregular shaped, and contained electron dense masses of particles that mixed with less dense aggregates and
lipid droplets could also be found. The matrix of these lysosomes was formed by fine, less osmiophilic particles. A few granules may resemble
those found in Dubin-Johnson syndrome. However, these granuleswere small in size and dense-cores are absent in them. Conclusion:
The specific lysosomes containing electron dense particles in hepatocytes is of great value for diagnosis of Gilbert's syndrome. |
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