| 孙怀美林伟王翔宇董艳王蓁△.宫颈癌中端粒酶的表达和高危型人乳头瘤病毒感染[J].,2012,12(18):3430-3435 |
| 宫颈癌中端粒酶的表达和高危型人乳头瘤病毒感染 |
| Expression of Telomerase and Infection of High Risk Human Papillpmavirusin Cervical Cancer |
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| DOI： |
| 中文关键词: 宫颈上皮内瘤变 宫颈肿瘤 免疫组化 端粒 末端转移酶 人乳头瘤病毒 |
| 英文关键词: Cervical Intraepithelial Neoplasia Cervix Neoplasms Immunohistochemistry Telomerase Human papilloma virus |
| 基金项目: |
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| 中文摘要: |
| 目的：研究不同程度子宫颈病变中高危型人乳头瘤病毒HR-HPV 感染和端粒酶活性的表达，以探讨两者在宫颈癌及宫颈上
皮内瘤变中的作用及相关性。方法：采用第二代杂交捕获技术检测宫颈脱落细胞HPV-DNA 含量，并用免疫组织化学EnVision 二
步法检测宫颈组织标本中端粒酶的表达。结果：（1） 端粒酶阳性表达率在对照组、CINⅠ、CINⅡ、CINⅢ和宫颈癌组分别为10.00
%、16.67 %、40.00 %、70.00 %、95.00 %，宫颈癌组高于CINⅢ，CINⅢ高于CINⅡ，CINⅡ高于CINⅠ，差异均有统计学意义（x2=4.
329，P＝0.037；x2=4.327，P＝0.038；x2=4.022，P＝0.045）。（2）随着宫颈病变级别的增加，高危型HPV 的阳性率和病毒负荷量均增
高。高危型HPV 的阳性率在宫颈癌和CINⅢ组明显高于对照组、CINⅠ及CINⅡ（x2=29.501~7.414，P＜0.01）。高危型HPV 的病毒
负荷量在对照组与其他4 组比较，差异均有统计学意义（P<0.05）；CINⅠ组分别与CINⅡ、CINⅢ及宫颈癌组比较差异均有统计学
意义（P<0.05）。（3）随着宫颈病变级别的增加，高危型HPV 的阳性率和端粒酶阳性表达率依次递增，两者有明显的相关性（r＝
0.943，P<0.01）。结论：高危型HPV 感染和端粒酶活性均与宫颈癌前病变及宫颈癌的发生发展密切相关，有望作为子宫颈癌前病
变和宫颈癌筛查的监测指标。 |
| 英文摘要: |
| Objective: To investigate the effect and relationship between high risk human papillomavirus (HR-HPV) infection and
telomerase expression in the development of cervical carcinoma. Methods: HPV DNA in cervical exfoliated cells was detected by second-
generation hybird capture technology, then the expression of telomerase was evaluated by immunohistochemistry. Results: (1) The
positive rate of telomerase in control group, cervical intraepithelial neoplasia(CIN)Ⅰ group, CINⅡ group, CINⅢ group and cervical carcinoma
group was 10.00 %, 16.67 %, 40.00 %, 70.00 % and 95.00 %, respectively. The positive rate of telomerase in cervical cancer was
significantly higher than that in CINⅢ (x2=4.329, P＝0.037). And the positive rate in CINⅢ was significantly higher than that in CINⅡ
(x2=4.327, P＝0.038). And the positive rate in CINⅡ was significantly higher than that in CINⅠ (x2=4.022, P＝0.045). (2) With the deterioration
of carcinoma lesions, HR-HPV load and the positive rate of HPV infection increased gradually. The positive rate of HPV infection
was significantly different between the cervical cancer group, CINⅢ group and CINⅡgroup, CINⅠgroup and control group
(x2=29.501~7.414, P＜0.01). The high risk HPV load was significantly different between control group and the other groups (P＜0.05).
(3) With the increase of the cervical lesion level, both the high-risk HPV detection rate and the telomerase positive expression rate increased
, and there was an obvious correlation between the HPV infection and the telomerase expression (r＝0.943, P<0.01). Conclusion:
High-risk type HPV infection and the expression of telomerase had an obvious correlation with the development of cervical intraepithelial
neoplasia and cervical cancer, and might be a monitoring index to detect and predict cervical cancer. |
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