| 罗湘玉郑雪松罗卫民△.穿心莲内酯对人肺癌细胞增殖以及侵袭相关分子表达的影响[J].,2012,12(17):3249-3252 |
| 穿心莲内酯对人肺癌细胞增殖以及侵袭相关分子表达的影响 |
| Effect of Andrographolide on Human Lung Cancer Cells Proliferationand Invasion-associated Molecular Expression |
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| DOI： |
| 中文关键词: 穿心莲内酯 肺癌细胞 Na+-K+-ATP 酶活性 蛋白激酶C 血管内皮生长因子 转化生长因子β1 |
| 英文关键词: Andrographolide Lung cancer cells Na+-K+-ATPase activity Protein kinase C Vascular endothelial growth factor Transforming growth factor β1 |
| 基金项目: |
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| 摘要点击次数: 768 |
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| 中文摘要: |
| 目的：观察穿心莲内酯(Andrographolide, AD)对人非小细胞肺癌细胞系H3255 细胞的生长抑制作用，并研究其对肿瘤生长
相关标记物血管内皮生长因子（vascular endothelial growth factor, VEGF）、转化生长因子（transforming growth factor，TGF）β1 的表
达以及蛋白激酶C（protein kinase C，PKC）的活性有无影响。方法：体外培养H3255 细胞，分别用1.0、2.5、5.0 μmol/L 的AD 在处
理细胞24h。MTT 法检测细胞的增殖，比色法检测乳酸脱氢酶（lactate dehydrogenase，LDH）的释放情况。ELISA 检测DNA 片段化
情况以及VEGF 和TGF-β1 的产生；无机磷法检测Na+-K+-ATP 酶活性以及磷基转移法测得PKC 活性。结果：AD 处理能呈剂量
依赖性方式降低H3255 细胞的活性以及Na+-K+-ATP 酶活性（P<0.05），同时也能促进LDH 的释放和DNA 片段的形成，并减少肺
癌细胞VEGF 和TGFβ1 的水平和PKC 的磷酸化。结论：AD 对肺癌细胞具有一定的抑制作用，有望成为一种潜在的肿瘤治疗药
物。 |
| 英文摘要: |
| Objective: To observe the growth suppression effect of Andrographolide (AD) on non-small cell lung cancer cell line
H3255, and to study the expression of tumor growth associated biomarkers such as vascular endothelial growth factor (VEGF)，transforming
growth factor β1 (TGF-β1) and the activity of protein kinase C (PKC). Methods：H3255 cells were cultured in vitro, and incubated
with 1.0, 2.5 or 5.0 μmol/L AD for 24h. MTT assay was performed to examine cell viability, LDH activity was determined by colorimetric
methods. DNA fragmentation, expression of VEGF and TGF-β1 were detected by ELISA. The ATPase activity and PKC activity were
tested by inorganic phosphorus detection method and phosphoryl abevacuation, repectively. Results：AD treatments concentration-dependently
decreased cell viability, and lowered Na+-K+-ATPase activity (P<0.05). This compound also increased DNA fragmentation and
LDH releasing. It also reduced VEGF and TGF-β1 levels, and inhibited protein kinase C activity in H3255 cells (P<0.05). Conclusion：
AD can inhibit lung cancer cells growth, and thus it may be a potent anti-lung cancer agent. |
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