| 徐沙沙1,2 项锋钢1,2△ 党受琴3 冉雯雯2 李宏2.NF-kB 与Slug 在非小细胞肺癌及其上皮间质转化中的作用[J].,2012,12(17): |
| NF-kB 与Slug 在非小细胞肺癌及其上皮间质转化中的作用 |
| The Expression of NF-kB and Slug in NSCLC and the Effect on EMT |
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| DOI： |
| 中文关键词: NF-kB Slug EMT RT-PCR 免疫组织化学 NSCLC |
| 英文关键词: NF-κB Slug EMT RT-PCR Immunohistochemistry |
| 基金项目: |
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| 中文摘要: |
| 目的：研究核因子NF-kB 与slug 在非小细胞肺癌（NSCLC）中的表达情况、及二者与非小细胞肺癌上皮间质转化（EMT）的
关系，为非小细胞肺癌的诊断治疗提供理论依据。方法：（1）采用免疫组化PV9000 二步法测定50 例NSCLC 组织及20 例相应正
常肺组织中NF-kBP65、slug、E-cadherin 及Vimentin 蛋白表达情况。（2）采用RT-PCR 测定其中25 例NSCLC 组织及10 例相应正
常肺组织中NF-kBP65、slug 的mRNA 表达情况。结果：NSCLC 中NF-kBP65 蛋白表达量高于癌旁正常肺组织（Z=-2.370, P＜
0.05），NF-kBP65mRNA 表达量明显高于癌旁正常肺组织（t=4.967,P＜0.01）；Slug 蛋白表达量明显高于癌旁正常肺组织（Z=-4.443,
P＜0.01），SlugmRNA 表达量明显高于癌旁正常肺组织（t=6.483,P＜0.01）。在NF-kBP65 阳性癌组织中，E-cadherin 蛋白表达下降
（x2=5.024, P＜0.05），Vimentin 蛋白表达上升（x2=4.723,P＜0.05）；Slug 阳性癌组织中，E-cadherin 蛋白表达下调（x2=5.984,P＜
0.05），Vimentin 表达上调（x2=5.028,P＜0.05）。另外，NF-kBP65 与Slug 在蛋白水平呈极显著正相关（r=0.443, P＜0.01），在mRNA
水平呈显著正相关（r=0.439,P＜0.05）。NF-kB 与分化程度（x2=5.024,P＜0.05）、有无淋巴结转移（x2=7.933, P＜0.01）及肿瘤的分期
（x2=5.614,P＜0.05）有关，与性别、年龄、组织类型无明显相关性（P＞0.05）；Slug 与淋巴结转移（x2=6.174, P＜0.05）及肿瘤的分期
（x2=7.317,P＜0.01）有关，与性别、年龄、组织类型、分化程度无明显相关性（P＞0.05）。结论：NF-kB、Slug 在NSCLC 中表达增强，可
能与NSCLC 的发生、发展、转移有关；并且NF-kB 与Slug 可能协同抑制E-cadherin 表达，促进Vimentin 表达，诱使NSCLC 的
EMT 发生，从而为进一步研究NSCLC 的EMT 提供理论依据。 |
| 英文摘要: |
| Objective: To investigate the expression of NF-kB and Slug in NSCLC and their relationship with EMT, to provide
theoretical basis to the treatment of NSCLC. Methods: (1) PV9000 two-step immunohistochemical method was used to detect the expression
of NF-κBP65, Slug, E-cadherin and Vimentin in 50 NSCLC tissues and 20 adjacent normal lung tissues. (2) RT-PCR was performed
to determine the expression of NF-κBP65mRNA and SlugmRNA in 25 NSCLC tissues and 10 adjacent normal lung tissues. Results: In
NSCLC tissues, NF-κBP65 expression was higher than that in adjacent normal lung tissues (Z=-2.370, P＜0.05); mRNA expression of
NF-kBP65 was significantly higher than that in adjacent normal lung tissues (t=4.967,P＜0.01); Slug expression was significantly higher
than that in adjacent normal lung tissues (Z=-4.443,P＜0.01); The expression of Slug was significantly higher than that in adjacent
normal lung tissues (t=6.483, P＜0.01). In NF-kBP65 positive NSCLC tissues, E-cadherin expression decreased (X2=5.024,P＜0.05)
while vimentin expression increased (X2=4.723,P＜0.05). In Slug positive NSCLC tissues, E-cadherin expression decreased (X2=5.984,
P＜0.05) and Vimentin expression increased (X2=5.028,P＜0.05). In addition, NF-kBP65 had very significantly positively correlated with
Slug (r=0.443,P＜0.01). NF-kB was related to differentiation degree (X2=5.024,P＜0.05), lymph node metastasis (X2=7.933, P＜0.01)
and neoplasm staging (X2=7.317,P＜0.01), but not related to gender, age and tissue type (P＞0.05). Slug was related to lymph node
metastasis (X2=6.174,P＜0.05) and neoplasm staging (X2=7.317,P＜0.01), but not related to gender, age, tissue type and differentiation
degree (P＞0.05). Conclusion: The increase of NF-kB expression and Slug expression in NSCLC may be related to the generation,
development and metastasis of NSCLC; in addition, NF-kB and Slug may synergistically inhibit E-cadherin expression, promote
Vimentin expression and induce EMT of NSCLC, which further provides theoretical basis to the researches on EMT of NSCLC. |
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