| 彭洁李文丽李玉亮海春旭.辐射所致小鼠肾脏氧化损伤及川芎嗪的保护作用[J].,2012,12(16):3064-3067 |
| 辐射所致小鼠肾脏氧化损伤及川芎嗪的保护作用 |
| Oxidative Stress Damage Induced by Irradiation in Mouse Kidney and theProtective Effect of Ligustrazine against it |
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| DOI： |
| 中文关键词: 川芎嗪 辐射 肾脏 抗氧化 |
| 英文关键词: Ligustrazine Irradiation Kidney Antioxidant |
| 基金项目:本项目得到长江学者，创新团队发展计划资助；国家自然科学基金资助（30872135；31170807） |
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| 中文摘要: |
| 目的：研究川芎嗪对辐射所致小鼠肾脏氧化损伤的预防和治疗作用。方法：采用60Co-γ 射线5 Gy 全身单次照射小鼠造模，
在照射前和照射后分别于每天腹腔注射川芎嗪130 mg/kg，连续给药10 d，进行预防和治疗，并设对照组，观察肾组织中丙二醛
（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）及总抗氧化力
（T-AOC）的变化。结果：与阴性对照组比较，照射可显著增加肾组织中MDA 的含量（P<0.05），降低SOD、CAT 的活性（P<0.05），
升高GSH-Px 活性（P<0.05），降低GSH 含量（P<0.05），使肾组织T-AOC 下降（P<0.05），。与照射组比较，给予川芎嗪预防和治疗
后，均可降低肾组织MDA 含量（P<0.05），升高肾组织T-AOC（P<0.05），且治疗组优于预防组，与阴性对照组无显著性差异。同时，
预防组可使SOD 活性和GSH 含量升高（P<0.05），治疗组可使SOD 和CAT 活性增高（P<0.05），但均对GSH-Px 活性无显著影响
（P>0.05）。结论：川芎嗪具有很好的抗氧化作用，无论预防和治疗均可降低辐射所致小鼠肾脏的氧化应激损伤，并且治疗效果优于
预防效果。 |
| 英文摘要: |
| Objective: To investigate the oxidative stress damage induced by irradiation in mouse kidney and the preventive and
therapeutic effect of Ligustrazine against it. Methods: Mice were exposed to 5Gy single 60Co-γ irradiation to induce oxidative stress, the
concentration of Ligustrazine for intraperitoneal is 130 mg/kg to prevent and treat liver injury before and after irradiation, The injection
lasted 10 days. The parameters of malondialdehyde（MDA）, superoxide dismutase enzyme (SOD),catalase (CAT), reduced glutathione
hormone(GSH), glutathione peroxidase (GPx), total antioxidation capability (T-AOC)activity in kidney were measured. Results: Compared
with that in the normal control group, irradiation significantly increased kidney content of MDA (P<0.05), decreased CAT, SOD
activity (P<0.05), GSH-Px activity (P<0.05), while it decreased kidney content of GSH (P<0.05), the kidney tissue T-AOC decreased
(P<0.05). By use of Ligustrazine, MDA content decreased (P<0.05), T-AOC were significantly higher (P<0.05), CAT activity increased
(P<0.05) and the effect of treatment group was better than the prevention group had no significant difference (P<0.05) compared with that
in the normal group. Meanwhile, the activity of SOD and the content of GSH significant increased in prevention group (P<0.05); The activity
of SOD and CAT significant increased in treatment group (P<0.05); But GSH-Px activity was no significant difference either in
treatment group or in prevention group. Conclusion: The irradiation can cause oxidative stress damage in mouse kidney tissue, Ligustrazine
can reduce or reverse this damage both in treatment and prophylaxis, and treatment group is better than the prevention group. |
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