| 赵明1,2 纪霞1,2△ 王海燕2 贾少丹2 张伟毅3.外周血单个核细胞TNF-α 基因表达与哮喘相关表型*[J].,2012,12(15):2920-2924 |
| 外周血单个核细胞TNF-α 基因表达与哮喘相关表型* |
| The Expression Levels of TNF-α mRNA in Peripheral Blood MononuclearCells with Asthma-related Phenotypes* |
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| DOI： |
| 中文关键词: 哮喘 TNF-α mRNA PBMC 实时荧光定量PCR 表型 |
| 英文关键词: Asthma TNF-α mRNA PBMC Real time quantitative PCR Phenotype |
| 基金项目:山东省卫生科技发展计划项目；青岛市科技计划基础研究项目（11-2-4-7-(2)-jch）；
青岛市市南区科技发展计划项目（12009-4-31-GG） |
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| 中文摘要: |
| 目的：探讨外周血单个核细胞（PBMC）TNF-α 基因mRNA 表达与哮喘严重程度、临床病理及相关影响因素的关系。方法：采
用实时荧光定量PCR 技术检测67 例哮喘病人和25 例健康对照PBMC 中TNF-α mRNA 表达水平，分析其与哮喘控制程度、血
浆TNF-α 浓度、嗜酸性粒细胞百分比（EOS%）、血浆总IgE 浓度和哮喘相关影响因素的关系。结果：经方差分析和SNK-q 检验，哮
喘未控制组PBMC 中TNF-α mRNA 表达水平高于正常组（P < 0.01）、控制组（P < 0.01）和部分控制组（P < 0.05）。相关分析显示哮
喘病人TNF-α mRNA 表达与血浆TNF-α 浓度和EOS%呈正相关，相关系数分别为r = 0.584（P <0.01）和r = 0.29（P < 0.05），有吸
烟史的哮喘病人TNF-αmRNA 表达水平高于非吸烟病人（P < 0.05）。结论：哮喘病人PBMC 中TNF-α mRNA 表达水平与哮喘的
控制程度呈负相关，与血浆TNF-α 浓度呈正相关，PBMC 中TNF-α mRNA 表达水平与血浆TNF-α 浓度可作为哮喘控制程度的
参考指标。 |
| 英文摘要: |
| Objective: To observe the association of TNF-α mRNA expression in peripheral blood mononuclear cells (PBMC)
with asthma severity, clinicopathology and asthma-related infuence factors. Methods: The relative expression levels of TNF-α mRNA in
PBMC were detected by real time fluorescence quantitive PCR from 67 asthma patients with diverse severities and 25 healthy controls.
The results were compared among groups and the correlation analysis were performed between the expression levels of TNF-α mRNA in
PBMC with the levels of plasma TNF-α, the percentage of peripheral blood eosinophils (EOS%), the levels of total plasma IgE, and
asthma-related influence factors. Results: The relative expression levels of TNF-α mRNA in PBMC in uncontrolled asthma patients were
higher than that in healthy controls (P < 0.01), controlled asthma patients (P < 0.01), and partly controlled asthma patients (P < 0.05).
Pearson product-moment correlation analysis showed that TNF-α mRNA expression in PBMC were correlated with plasma TNF-α and
EOS% with r = 0.584 (P < 0.01) and r = 0.29 (P < 0.05) respectively. The relative expression levels of TNF-α mRNA in patients with
positive smoking history were higher than those in patients with no smoking history. Conclusion: The relative expression levels of
TNF-α mRNA in PBMC were negatively correlated to the levels of asthma control, and were positively correlated to plasma TNF-α.
Both the expression levels of TNF-α mRNA in PBMC and plasma TNF-α could be potential makers for management of asthma. |
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