| 黄杰 宋宇 徐汇川 李凤玲 栾天竹 周立君.三氧化二砷对人脐静脉内皮细胞凋亡及VCAM-1/ICAM-1
表达的影响[J].,2012,12(14):2647-2650 |
| 三氧化二砷对人脐静脉内皮细胞凋亡及VCAM-1/ICAM-1
表达的影响 |
| Influences of Arsenic Trioxide on Proliferation/Apoptosis and Expressionof ICAM-1/VCAM-1 of Human Umbilical Vein Endothelial Cells |
| |
| DOI： |
| 中文关键词: 三氧化二砷 人脐静脉内皮细胞 凋亡 VCAM-1 ICAM-1 |
| 英文关键词: Arsenic trioxide Human umbilical vein endothelial cells Apoptosis Intercellular adhesion molecule-1 Vascular cell
adhesion molecule-1 |
| 基金项目: |
|
| 摘要点击次数: 682 |
| 全文下载次数: 817 |
| 中文摘要: |
| 目的：观察三氧化二砷（As2O3）对血管内皮细胞增殖、凋亡及VCAM-1/ICAM-1 表达的影响，探讨As2O3 对血管内皮细胞增
殖生长以及炎症反应的影响。方法：人脐静脉内皮细胞（HUVEC）体外培养，以不同As2O3 浓度及时间对其进行干预。采用CCK-8
测定细胞增殖活性，流式细胞仪AnnexinⅤ/PI 双染法检测细胞的凋亡率，实时荧光定量PCR 检测VCAM-1mRNA 表达，酶联免
疫吸附试验（ELISA）检测细胞间黏附分子（VCAM-1）及血管细胞黏附分子（ICAM-1）的表达情况。结果：当As2O3 浓度在3 μmol·
L- 1 时HUVEC 培养24 h 的的凋亡率为（0.134±0.03）%，48 h 为（3.305±0.53）%，72 h 为（3.748±0.84）%（P<0.05），凋亡率均在一
较低水平。当As2O3 浓度>3 μmol·L- 1 时HUVEC 凋亡率明显增加（P<0.01）。不同浓度As2O3 作用HUVEC48 h 后检测上清液中
ICAM-1 与VCAM-1 浓度时发现1 μmol·L- 1 时VCAM-1 表达即开始增加（123.32±3.78 mmol·L- 1，P<0.01）,
而HUVEC 表达
ICAM-1 含量与对照组相比差异并不明显（38.94±2.59 mmol·L- 1，P>0.05）， 随着As2O3 浓度的增加，HUVEC 表达
ICAM-1/VCAM-1 的量均增加但敏感性不同。对照组及(1.0、2.0、3.0、4.0、5.0) μmol·L- 1 As2O3 作用于HUVEC 48 h 实时荧光定量
PCR 法检测VCAM-1mRNA 表达量明显增加，与对照组相比实验组的表达量分别为（1.657±0.287,1.858±0.241,2.321±
0.280,3.012±0.235,3.508±0.342）(P<0.01)。结论：As2O3 可直接降低细胞活性，诱导细胞凋亡，并且呈一定的时间- 浓度依赖性。
在较低浓度时VCAM-1/ICAM-1 的表达在一个相对较低的水平，随着As2O3 浓度的逐渐升高，内皮细胞凋亡率增高，
VCAM-1/ICAM-1 表达增加，并且VCAM-1/ICAM-1 对As2O3 的敏感性呈现一定的差异性。 |
| 英文摘要: |
| Objective: To investigate the effects of arsenic trioxide (As2O3) on the proliferation and apoptosis of endothelial cells
and the influences of As2O3 on the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1)
in vitro. Methods: Human umbilical vein endothelial cells (HUVEC) were incubated in medium with different concentration of As2O3.
The proliferation profile of HUVEC was determined by CCK8 Kit. The apoptosis of HUVEC was detected by microscopy and flow
cytometry (FCM). The expression of ICAM-1/VCAM-1 was detected by applying ELISA and Real-time PCR. Results: The apoptosis rate
of HUVEC was in a low level valued（0.134±0.03）%,（3.305±0.53）%,（3.748±0.84）%（P<0.05）when incubated in medium with 3
μmol·L-1 of As2O3 for 24 h, 48 h and 72 h. When the concentration of As2O3 was more than 3 μmol·L-1, the apoptosis rate of HUVEC
promoted significantly (P<0.01). The outcomes of ELISA showed that, compared with that in control group, the expression of VCAM-1
increased significantly (valued 123.32±3.78 mmol·L-1, P<0.01), while the expression of ICAM-1 did not change (P>0.05), when
incubated in medium with 1.0 μmol·L-1 of As2O3. With the increasing of the concentration of As2O3, the reaction of ICAM-1/VCAM-1 to
As2O3 was different. The expression of VCAM-1 mRNA (fold of change) were （1.657±0.287,1.858±0.241, 2.321±0.280, 3.012±
0.235, 3.508±0.342）(P<0.01) in 1.0, 2.0, 3.0 4.0 5.0 μmol·L-1 groups, respectively. Conclusion: Arsenic trioxide can promote the
apoptosis of HUVEC, increase the expression of VCAM-1/ICAM-1 in a dosage and time dependent manner. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
