| 程晓辉 徐学森 岳子勇 公维东 王楠.高碳酸血症对大鼠机械通气肺损伤时炎症因子和p38MAPK
表达的影响[J].,2012,12(14):2605-2608 |
| 高碳酸血症对大鼠机械通气肺损伤时炎症因子和p38MAPK
表达的影响 |
| Effects of Hypercapnia on Expression of Inflammatory Cytokinesand p38MAPK in Ventilator-Induced Lung Injury in Rats |
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| DOI： |
| 中文关键词: 高碳酸血症 机械通气肺损伤 p38MAPK |
| 英文关键词: Hypercapnia Ventilator-induce lung injury P38MAPK |
| 基金项目:国家自然科学基金项目(30772085) |
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| 中文摘要: |
| 目的：探讨高碳酸血症对大鼠机械通气性肺损伤（VILI）时炎症因子和p38MAPK 表达的影响。方法：健康雄性Wistar 大鼠
30 只，体重220～280g，采用随机数字表法，将大鼠随机分3 组（n=10)：对照组（C 组）、机械通气肺损伤组（V 组）和高碳酸血症组
(H 组)。C 组保留自主呼吸，V 组和H 组行机械通气4 h。采用高气道压机械通气模式制备机械通气性肺损伤模型。H 组通过调整
吸入的CO2 浓度来维持动脉血PaCO2 分别为80～100mmHg。机械通气结束时，测定支气管肺泡灌洗液(BALF)中总蛋白、TNF-α
和巨噬细胞炎症蛋白-2（MIP-2）的浓度；取肺组织，测定湿干重比（W/D 比）、细胞间粘附分子（ICAM-1）和p38MAPK 蛋白的表达
水平以及p38MAPK 的活性，并观察病理学结果，进行肺损伤评分。结果：与C 组比较，V 组肺损伤评分、W/D 比、ICAM-1 表达水
平、BALF 中总蛋白浓度、TNF-α 和MIP-2 浓度和肺组织p38MAPK 活性升高，PaO2 降低（P<0.05）；与V 组比较，H 组肺损伤评分、
W/D 比、ICAM-1 表达水平、BALF 中总蛋白浓度、TNF-α 和MIP-2 浓度和肺组织p38MAPK 活性降低，PaO2 升高（P<0.05）。结论：
高碳酸血症通过调节p38MAPK 的表达，从而抑制炎症反应减轻大鼠机械通气肺损伤。 |
| 英文摘要: |
| Objective: To investigate the effects of hypercapnia on the inflammatory cytokines and p38MAPK in ventilator lung
injury in rats. Methods: Thirty male Wistar rats weighting 250～280g were randomly divided into 3 groups (n=10 each):control group
(group C),VILI group(group V), hypercapnia group(group H).A rat model of lung injury induced by ventilation with high peak inspiratory
pressure(PIP). Parameter of ventilation: PIP=25cmH2O, PEEP=2cmH2O, the fraction of inspiratory O2 50% and adjusting the level of CO2
to maintained PaCO2 of group V and group H at 35～45mmHg and 80～100mmHg respectively. The rats were sacrificed by exsanguination
4h of mechanical ventilation and the lung tissues were removed for microscopic examination, lung wet to dry weight ratio(W/D), determination
of ICMA-1(by immuno-histochemistry), p38 and phosphorylated p38(p-p38) expresstion (byWestern blotting) and determination
of TNF-α, MIP-2 (by enzyme linked immunosorbent assay) and total of protein concentration in bronchoalveolar lavage fluid(BALF).
Results: Compared with group C, the lung injury score, W/D ratio, TNF-α, MIP-2,total protein concentrations in BALF, ICAM-1 expression
and p38MAPK activity in lung tissue were increased, PaO2 were decreased in groups V (P<0.05). Compared with group V, the lung
injury score, W/D ratio, TNF-α, MIP-2, total protein concentrations in BALF, and MAD content, ICAM-1 expression and p38MAPK
activity in lung tissue were decreased, PaO2 and were increased in group P (P<0.05). Conclusions: Hypercapnia attenuated
ventilator-induce lung injury by regulating the expression of p-p38MAPK that inhibit the expression of inflammatory cytokines in rat lung
tissues. |
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