| 唐利1 刘博强1 简强1 廖文俊1 迟素敏2 李承新1△.Rab23 在鳞癌细胞系中的表达及其对鳞癌细胞侵袭的影响*[J].,2012,12(13):2440-2443 |
| Rab23 在鳞癌细胞系中的表达及其对鳞癌细胞侵袭的影响* |
| The Expression of Rab23 in Squamous Cell Carcinoma Cell Lines and ItsRole on the Cell Invasion of SCC |
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| DOI： |
| 中文关键词: Rab23 鳞癌 侵袭 |
| 英文关键词: Rab23 Squamous Cell Carcinoma Invasion |
| 基金项目:国家自然科学基金项目（30872264） |
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| 中文摘要: |
| 目的：观察Rab23 在鳞癌细胞系中的表达及其对鳞癌细胞侵袭的影响。方法：western blot 检测Rab23 在四个头颈部鳞癌细
胞系中的表达，对鳞癌细胞系HSQ-89 用脂质体转染小干扰RNA，对Sa3 用脂质体转染Rab23 质粒，分别干涉或过表达Rab23，
用铺有基质胶的transwell 小室检测细胞侵袭能力，细胞划痕试验检测迁移能力。结果：Rab23 在四个鳞癌细胞系中均有表达，在
Hacat 中不表达，HSQ-89 细胞经Rab23 干涉后细胞侵袭能力减弱，而Sa-3 细胞过表达Rab23 后细胞侵袭能力增强。干涉或过表
达Rab23 后，鳞癌细胞的迁移能力无差异。结论：Rab23 可促进鳞癌细胞侵袭。 |
| 英文摘要: |
| Objective: To observe the expression of Rab23 in Squamous Cell Carcinoma (SCC) cell lines and its role on the cell
invasion of SCC. Methods: The expression of Rab23 in four SCC cell lines was detected by western blot. RAB23 siRNAs or RAB23
cDNA were transfected by Oligofectamine in Hsq-89 cells or Sa-3 cells respectively. Biocoat Matrigel invasion chambers were used to
detect the invasion ability. Result: Rab23 expression was found in all SCC cell lines except for Hacat. Cellular invasion was significantly
reduced by siRNA silencing of RAB23 in Hsq-89 cells, whereas significantly enhanced by overexpression of RAB23 in Sa-3 cells.
Silencing or overexpression of Rab23 had no effect on SCC cell migration. Conclusion: Rab23 can enhance the invasion of SCC cells. |
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