| 何翠瑶1，2 谷容1 张翼冠2 李晓辉2△.正交设计筛选三七皂苷单体最佳组合及对血管内皮保护作用评价[J].,2012,12(13):2410-2413 |
| 正交设计筛选三七皂苷单体最佳组合及对血管内皮保护作用评价 |
| Optimization of Monomer Combination of Panax Notogiseng Saponins byOrthogonal Design and Evaluation of the Potective Effect on VascularEndothelial Cell |
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| DOI： |
| 中文关键词: 三七皂苷 内皮细胞 正交实验 |
| 英文关键词: Panax Notogiseng Saponins Endothelial cell Orthogonal experiment |
| 基金项目:国家自然科学基金资助项目（304770465，30672461），重庆卫生局课题（2009-2-95） |
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| 中文摘要: |
| 目的：利用正交实验筛选出三七皂苷单体最佳组合并观察其对氧化低密度脂蛋白（Ox-LDL）损伤血管内皮细胞的保护作用。
方法：体外培养人脐静脉内皮细胞（HUVEC），加入100mg·L-1 OX -LDL 诱导单核- 内皮细胞黏附，蛋白染料法测定黏附值，通过
正交试验设计筛选出三七皂苷单体最佳组合；比色法检测乳酸脱氢酶（LDH）的释放量；硝酸还原法和放射免疫法分别测定HUVEC
培养上清液中一氧化氮(NO)、内皮素(ET)含量。结果：获得最佳PNS 单体组合为：Rg1：Rb1：R1 为1：10：1（Rg1 10-5mol/L、Rb1
10-4mol/L、R1 10-5mol/L)，最佳单体组合显著降低LDH 及ET 水平，提高NO 含量（P<0.01）。结论：由正交实验设计筛选出的最佳
单体组合对OX-LDL 所致的血管内皮细胞损伤有显著的保护作用。 |
| 英文摘要: |
| Objective: To select the optimal monomer combination by orthogonal experiment and evaluate the protective effect of
the monomer combination on vascular endothelial cell. Methods: The human umbilical vein endothelail cells (HUVECs) cultured in vitro
were treated wih 100 mg·L-1 Ox-LDL to induced the adhension between the monocyte and endotheliocyte, the adhesion rate was measured
by dye-bond protein method. Select the effective monomer combination by orthogonal experiment; the release amount of LDH was
determined by colourimetry; the content of ET was determined by nitric-reductive assay and the content of NO was determined by radioimmunoassay.
Results: The best combination group was Rg1：Rb1：R1=1:10:1 (Rg1 10-5mol/L, Rb1 10-4mol/L, R1 10-5mol/L). Conclusions:
The best combination of the monomers could protect the VEC against damage. |
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