| 毕罡1 汪黎黎2 靳风烁1 李彦锋1 吴刚1 葛成国1.CPE 对体外培养前列腺癌细胞株的抑制作用[J].,2012,12(13):2401-2405 |
| CPE 对体外培养前列腺癌细胞株的抑制作用 |
| The Depressant Effections in the Growth of Prostate AdenocarcinomaCells with CPE in Vitro |
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| DOI： |
| 中文关键词: 产气荚膜梭菌肠毒素 前列腺癌 免疫荧光染色 Claudin-3 |
| 英文关键词: Clostridium perfringens enterotoxin Prostate adenocarcinoma Immunofluorescence Claudin-3 |
| 基金项目:国家自然科学基金资助（30400446） |
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| 中文摘要: |
| 目的：探讨产气荚膜梭菌肠毒素（CPE）对前列腺癌细胞株在体外培养过程中的抑制作用。方法：以细胞免疫荧光染色观察人
前列腺癌PC-3 细胞株和22RV1 细胞株表达Claudin-3 情况；并分别用生长曲线测定法及MTT 法等测定CPE 对PC-3 和22RV1
细胞活性的影响；分析CPE 对肿瘤的生长抑制作用。结果：应用Claudin-3 单抗进行免疫荧光染色，证实体外培养的人前列腺癌细
胞株22RV1 可以特异性高表达Claudin-3，而在PC-3 上有弱表达；用相关方法测定经过不同浓度的CPE 处理后的前列腺癌细胞
株22RV1 细胞增殖明显降低，有明显抑制作用；而对PC-3 则未产生明显细胞毒性作用。结论：CPE 在体外可通过特异性作用于
Claudin-3 高表达的前列腺癌细胞株对前列腺癌细胞产生抑制作用。 |
| 英文摘要: |
| Objective: To investigate the depressant effections in the growth of prostate adenocarcinoma cells with clostridium
perfringens enterotoxin (CPE) in vitro. Methods: The various expressions in the two prostate adenocarcinoma cell strains of PC-3 and
22RV1 were detected by cellular immunofluorescence. The depressant effections in the growth of cell strains of PC-3 and 22RV1 were
assayed by using CPE in vitro with methods of Growth curve and MTT assay. Then the results were analyzed. Results: There were highly
specific expressions of the claudin-3 in the cell strain 22RV1 and lowly in PC-3 by immunofluorescence with monoclonal antibody of
claudin-3. It was observed that the obviously depressant effections in the growth of the cell strain 22RV1 with CPE in vitro, and not in
PC-3. Conclusions: CPE can inhibit the growth of prostate adenocarcinoma cells which are highly specific expressions of the claudin-3 in
vitro. |
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