| 姜珊1,2 史明1 张琛1 胡耿瑶1 赵钢1△.三七素对谷氨酸损伤的PC12 细胞的影响[J].,2012,12(12):2272-2274 |
| 三七素对谷氨酸损伤的PC12 细胞的影响 |
| The Effects of Dencichine on PC12 Cells Injuried by Glutamate |
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| DOI： |
| 中文关键词: 三七素 PC12 细胞 谷氨酸 |
| 英文关键词: Dencichine PC12 cell Glutamate |
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| 中文摘要: |
| 目的：研究三七素对谷氨酸损伤的PC12 细胞的影响。方法：用谷氨酸复制体外培养的PC12 细胞损伤模型，采用MTT 法、
Hoechst33342/PI 双重染色法分别研究高、低剂量三七素对谷氨酸所致的PC12 细胞损伤的影响。结果：谷氨酸刺激后，PC12 细胞
存活率较对照组显著降低（P<0.05）、凋亡显著增强（P<0.05）。给予高剂量三七素可加重谷氨酸引起的PC12 增殖力降低、凋亡增
强。但低剂量三七素干预后，细胞存活率较模型组显著升高（P<0.05），凋亡较模型组明显降低（P<0.05）。结论：高剂量三七素可加
重谷氨酸对PC12 细胞的损伤，但低剂量三七素可显著减轻谷氨酸对PC12 细胞的损伤，但具体机制尚有待于进一步研究。 |
| 英文摘要: |
| Objective: To investigate the effect of dencichine on PC12 cells injuried by glutamate. Methods: PC12 cells were
injuried by glutamate, and then the cells were theraped respectively by dencichine at the doses of 0.5 mg/kg and 0.5 mg/kg. The
proliferative ability and apoptosis of PC12 cells were detected by MTT and Hoechst33342/PI. Results: Dencichine at the dose of 2.5
mg/kg aggravated the inhibition of proliferative ability and apoptosis of PC12 cells induced by glutamate, but dencichine at the dose of
0.5 mg/kg ameliorated the inhibition of proliferative ability and apoptosis of PC12 cells induced by glutamate. Conclusions: Dencichine
is neurotoxic, dencichine and low dose of which could ameliorated the impairment caused by glutamate. |
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