| 张秋霞孔心涓△ 姜英俊徐永红李新华武军.Notch 通路在大鼠肝纤维化发生发展中作用的初探[J].,2012,12(11):2046-2048 |
| Notch 通路在大鼠肝纤维化发生发展中作用的初探 |
| Effects of Notch Signaling in Rats with Hepatic Fibrosis |
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| DOI： |
| 中文关键词: 肝纤维化 Notch 通路 上皮间充质转化 转化生长因子β1 |
| 英文关键词: Hepatic fibrosis Notch signaling Epithelium-mesenchymal transition Transforming growth |
| 基金项目: |
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| 中文摘要: |
| 目的：研究Notch 通路在肝纤维发生发展中作用及可能的分子机制。方法：Wistar 大鼠40 只随机分为正常对照组与病理
模型组，病理模型组皮下注射四氯化碳制备肝纤维化模型。8 周后将大鼠处死，取肝组织行病理HE 染色评价肝纤维化程度并采
用免疫组织化学法检测Notch-1 蛋白、E-cadherin 蛋白与TGF-β1 蛋白的表达。结果：肝组织病理HE 染色示肝纤维化大鼠肝脏肝
细胞坏死、再生明显，胶原纤维沉积明显增加，肝实质结构紊乱。与正常对照组相比，病理模型组notch-1 与TGF-β1 蛋白表达明显
增加，而E-cadherin 蛋白的表达明显下降（P <0.01）。结论：Notch 通路在大鼠肝纤维化发生发展中可能起重要作用。 |
| 英文摘要: |
| Objective: To investigate the effect of Notch signaling on the progression of hepatic fibrosis in a rat model induced by
CCl4. Methods: Forty healthy wistar rats were randomly divided into 2 groups: control group (n =15) and liver fibrosis group(n=25).
Experimental liver fibrosis was induced by subcutaneous injection of CCl4. At the end of 8 weeks, histopathological study of liver tissue
was done with hematoxylin-eosin (HE). Notch-1, E-cadherin and Transforming growth factor-β1(TGF-β1) in liver were examined with
the immunohisochemistrical technique. Results: A significant collagen deposition, hepatocyte necrosis and rearrangement of the
parenchyma were noted in liver tissue of liver fibrosis group. The expression levels of notch-1and TGF-β1 increased in model group than
those in control group. The expression levels of E-cadherin increased in the model animals (P<0.01). Conclusions: Notch signaling play
important role in the occurrence and progression of hepatic fibrosis in a rat model induced by CCl4, Notch signaling and TGF-β signaling
may have many consistency in the occurrence and progression of hepatic fibrosis. |
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