| 王彬1 朱敏1 王枫1 郑刚2 谭庆荣3 张雪平1△.ω-3 多不饱和脂肪酸对PTSD-SPS 大鼠空间学习记忆和海马神经元
溶酶体损伤的防护作用研究[J].,2012,12(11):2010-2013 |
| ω-3 多不饱和脂肪酸对PTSD-SPS 大鼠空间学习记忆和海马神经元
溶酶体损伤的防护作用研究 |
| Protective Effects of ω-3 Polyunsaturated Fatty Acids on PTSD-inducedImpairment of Spatial Learning and Memory and the Lysosomes inHippocampal Neurons |
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| DOI： |
| 中文关键词: PTSD ω-3 多不饱和脂肪酸 学习记忆 海马 Morris 水迷宫 |
| 英文关键词: PTSD Learning and memory ω-3 polyunsaturated fatty acids Hippocampus Morris Water Maze |
| 基金项目:国家自然科学基金(30972428) |
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| 中文摘要: |
| 目的：观察饲料中添加ω-3 多不饱和脂肪酸对PTSD-SPS 大鼠空间学习记忆能力及海马神经元溶酶体损伤的保护作用。方
法：将48 只健康成年雄性SD 大鼠随机分为正常对照组、PTSD-SPS 模型组、30 % ω-3PUFAs +PTSD-SPS 模型组、60 % ω-3PUFAs
+ PTSD-SPS 模型组。采用Morris 水迷宫测试方法，观察大鼠定位航行实验中逃避潜伏期及空间探索实验中靶象限活动时间
的百分比及穿台次数。电子显微镜观察大鼠海马神经元超微结构变化。结果：与对照组相比，SPS 模型组大鼠逃避潜伏期延长，第
5 天达到（39.12 ±7.34）s（P<0.05）；第6 天大鼠靶象限内活动时间百分比明显缩短及穿台次数减少，分别是10.01 % ± 3.03 %及
（1.05 ±0.13）次；与SPS 模型组对比，喂饲60 % ω-3PUFAs 的SPS 组大鼠逃避潜伏期为（19.13 ± 4.26）s（P<0.05），靶象限内活动
时间百分比及穿台次数为25.56 %±2.13 %、（2.36±0.34）次（P<0.05）。电镜结果显示，喂饲ω-3PUFAs 的SPS 模型组大鼠海马神
经元中溶酶体数量比SPS 组明显减少，与对照组没有显著差异。结论：ω-3 多不饱和脂肪酸可能通过减少海马神经元溶酶体的数
量对PTSD-SPS 大鼠学习记忆损伤起到一定的防护作用。 |
| 英文摘要: |
| Objective: To investigate the effects of ω-3 polyunsaturated fatty acids on PTSD-induced impairment of spatial learning
and memory and the lysosomes in hippocampal neurons. Methods: Forty-eight male SD rats were randomly and equally divided into
4 groups: control group, PTSD-SPS group, 30 % ω-3PUFAs + PTSD-SPS group, 60 % ω-3PUFAs + PTSD-SPS group. The escape latency
time was evaluated by using the navigation test in a Morris water maze, and the ratio of time spent in the target quadrant and the
number crossing plate was evaluated by the probe test. Ultrastructural changes of hippocampal neurons were detected by transmission
electron microscope. Results: Compared with the control group, the PTSD-SPS group had prolonged latency, (39.12 ±7.34) s（P<0.05）
in the fifth day, and had a decrease in the ratio of time spent in the target quadrant and the number of crossing plate in the sixth day, 10.01
%±3.03 % and （1.05±0.13）times, respectively. The mean escape latency of the rats in 60 % ω-3PUFAs group was （19.13 ±4.26）s
（P<0.05）, and the ratio of time spent in the target quadrant and the number of crossing plate was 25.56 % ±2.13 %,（2.36 ±0.34）times
（P<0.05）, respectively. Compared with the PTSD-SPS group, the number of lysosomes in the hippocampal neurons in 60 % ω-3PUFAs
group was fewer than that in the SPS group by transmission electron microscope. There was no significance difference be- tween the
ω-3PUFAs group and control group. Conclusion: Dietary Supplement of ω-3PUFAs may protect PTSD-induced impairment of spatial
learning and memory by decreasing the number of lysosomes in hippocampal neurons. |
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