| 夏宁1 高媛2 朱春红3 王兴1 刘卫辉1 王涛1 李海民1△.miR-200a 及AFP 在肝癌、肝硬化患者血清中表达比较分析*[J].,2012,12(9):1668-1670 |
| miR-200a 及AFP 在肝癌、肝硬化患者血清中表达比较分析* |
| A Comparison Analysis between the Expressions of Serum MiR-200a andAFP in Liver Cirrhosis and Liver Cancer Patients |
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| DOI： |
| 中文关键词: 肝癌 血清 早期诊断 miR-200a AFP |
| 英文关键词: Carcinoma, hepatocellular Serum Early diagnosis MiR-200a AFP |
| 基金项目:国家自然科学基金（81172061），陕西省自然科学基金（2011K3-03-04） |
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| 中文摘要: |
| 目的：分析miR-200a 及AFP 在肝癌、肝硬化患者血清中的表达水平并进行比较，探索其成为肝癌早期诊断血清标志物的可
能性。方法：临床收集肝正常、肝硬化、肝癌患者血液标本。运用实时定量PCR 技术检测血清miR-200a 的相对表达情况，血清AFP
水平从临床资料中提取。结果：临床标本分析结果显示，miR-200a 在肝硬化及肝癌患者中均显著下调（P<0.05），AFP 仅在肝癌患
者中出现异常表达。结论：血清miR-200a 极大程度地参与了肝癌发生，对肝硬化及肝癌具有一定的诊断价值。 |
| 英文摘要: |
| Objective: This study aimed to analyze the expression levels of serum microRNA-200a (miR-200a) and AFP in liver
cirrhosis and liver cancer patients, and to explore whether miR-200a could be a novel biomarker of liver cancer. Methods: Patients with
normal liver, liver cirrhosis and liver cancer in clinic were included in this study. Blood samples of the patients were collected for analysis.
Real time quantitative PCR technique was used to measure the expression level of serum miR-200a. The AFP expression was got from
clinical data. Results: Comparing with normal liver patients, miR-200a presented lower expressions in liver cirrhosis patients and liver
cancer patients (P<0.05), while AFP presented higher expressions only in liver cancer patients(P<0.05). Conclusion: Data indicated that
MiR-200a played an important role in liver cancer development and had diagnostic value for indicating liver cirrhosis and liver cancer.
Key words: Carcinoma, hepatocellular; Serum; Early diagnosis; MiR-200a; AFP |
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