| 蒲乾坤周催车会莲△.氨基葡萄糖对SD 大鼠肝脏糖代谢相关蛋白表达差异性的研究[J].,2012,12(9):1618-1620 |
| 氨基葡萄糖对SD 大鼠肝脏糖代谢相关蛋白表达差异性的研究 |
| The Effects of Glucosamine on Related Proteins of GlucoseMetabolism in Rats |
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| DOI： |
| 中文关键词: 氨基葡萄糖 免疫组化 糖代谢相关蛋白 |
| 英文关键词: GlcN Immunelhistochemistry Related proteins of glucose metablism |
| 基金项目:国家自然科学基金资助项目（30700662） |
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| 中文摘要: |
| 目的：研究氨基葡萄糖对SD 大鼠肝脏中糖代谢相关蛋白表达的影响。方法：选取成年雌性SD 大鼠48 只，按体重随机分成
4 组，分别为氨基葡萄糖高、中、低剂量组和阴性对照组。分别灌胃500mg/Kg，250mg/Kg，125mg/Kg 的氨基葡萄糖溶液和
1ml/100g 的生理盐水，连续灌胃28 天。实验结束时处死试验动物，用免疫组织化学法检测肝脏中蛋白激酶，己糖激酶，一氧化氮
合酶，葡萄糖转运蛋白4 和葡萄糖转运蛋白2 的表达情况。结果：试验期间各组动物生长发育情况良好，灌胃不同剂量氨基葡萄
糖的大鼠肝脏中五种蛋白的表达与阴性对照组相比均无显著性差异(P≥0.05)。结论：氨基葡萄糖未导致肝脏中糖代谢相关蛋白表
达的异常，提示服用氨基葡萄糖不会影响肝脏的糖代谢过程。 |
| 英文摘要: |
| Objective: To research the effects of glucosamine to glucose metabolism in liver through a 28-day feeding study with
SD rats. Methods: Forty-eight adult rats were randomly divided into four groups by their weights: one H group, one M group, one L group
and one C group. All rats were oral administrated with either normal saline or GlcN via gavage. The H group, M group, L group were fed
GlcN of 500mg/Kg, 250mg/Kg, 125mg/Kg respectively and the C group were fed normal saline of 1ml/100g every day. The rats were fed
for 28 days. At the end of the 28th day, five hepatic physiological indices of these rats were measured by immunohistochemistry. Results:
During the experiment, conditions of rats in each group were good. The rats of H, M and L group had normal hepatic physiological
indices and no organ tissue damage was detected. Conclusion: From the results of this 28 days feeding test, we can conclude that feed
different doses of GlcN to rats will not affect the metabolism of glucose in their liver. |
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