| 梁洪雨1,2 张晓宇1,2 邵宁一2 Philipp Khaitovich2△ 赵敏△.利用激光显微切割与microarray 技术对恒河猴脑中白质与
灰质基因表达的差异性的研究[J].,2012,12(9):1605-1608 |
| 利用激光显微切割与microarray 技术对恒河猴脑中白质与
灰质基因表达的差异性的研究 |
| Research of Differential Expression between GreyMatter and White Matterin Rhesus Macaque Brain by Laser Capture Microdessection and Microarray |
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| DOI： |
| 中文关键词: 激光纤维切割 灰质 白质 微芯片 |
| 英文关键词: Llaser capture microdissection(LCM) Grey matter White matter Array |
| 基金项目:中国科学院重要方向项目（KSCX2-EW-R-02-02）资助 |
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| 中文摘要: |
| 目的：利用激光显微切割技术和microarray 技术比较恒河猴脑组织中前额叶皮质（prefontal cortex, PFC）与小脑皮质（cerebellar
cortex, CBC）的灰质与白质基因表达的差异。方法：利用激光显微切割技术（laser capture dissection, LCM）与microarray 技术
的有效结合，提取恒河猴PFC 与CBC 的白质与灰质，分别提取RNA，合成cDNA 文库。最后利用GeneChip 1.0 ST 芯片技术，分
析得出大脑与小脑中灰质与白质的表达差异性。结果：无论是灰质还是白质，在PFC 中的高表达基因都要远远多于在CBC 中的
高表达基因。结论：使用LCM 可以提取单一的细胞群，从而用于要求更为精确的实验当中。 |
| 英文摘要: |
| Objective: To investigate the different expression of the genes in the gray matter,white matter and cerebellar cortex.
Methods: The white matter and gray matter were extracted from PFC and CBC via LCM technology. The expression differences between
gray matter and white matter in the PFC and CBC were analyzed. Results: There were more highly expressed genes in the PFC than in the
CBC whatever for grey matter or white matter. Conclusion: The LCM can extract a single cell population, which is vital for studies requiring
a high level of precision. |
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