| 梁少双魏良洲△ 荆雪田字彬孔心涓.下丘脑室旁核注射GLP-1 对糖尿病大鼠中枢GLP-1 受体表达[J].,2012,12(8):1452-1456 |
| 下丘脑室旁核注射GLP-1 对糖尿病大鼠中枢GLP-1 受体表达 |
| Effection of GLP-1 Injection in Paraventricular Nucleuson the Expression of GLP-1 Receptor and GastricEmptying in Diabetic Rats |
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| DOI： |
| 中文关键词: 糖尿病 室旁核 胃排空 胰高血糖素样肽-1 受体 |
| 英文关键词: Diabetes mellitus Paraventricular nucleus Gastric emptying GLP-1 Receptor |
| 基金项目:国家自然科学基金项目资助（ZR2009CM105） |
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| 中文摘要: |
| 目的：探讨下丘脑室旁核(hypothalamic paraventricular nucleus，PVN)注射GLP-1（胰高血糖素样肽-1）对糖尿病大鼠胃排空
的影响及机制。方法：30 只Wistar 大鼠随机分为正常对照组（NC 组）、糖尿病组(DM 组)和GLP-1 干预组（GLP-1 组），每组各10
只。DM 组和GLP-1 组腹腔注射链脲佐菌素，三组大鼠均PVN 区埋置套管，恢复7d, GLP-1 组微量注射0.5μg/0.5μl 的GLP-1，NC
组和DM 组大鼠PVN 区微量注射等体积生理盐水。甲基纤维素- 酚红灌胃法检测胃排空；半定量RT-PCR 检测大鼠下丘脑
GLP-1RmRNA 的表达。结果：DM 组胃排空率较NC 组明显升高（P＜0.05）, GLP-1 组胃排空明显低于DM 组（P＜0.05）, GLP-1
组和NC 组差异无统计学意义（P＞0.05）。GLP-1 组下丘脑GLP-1RmRNA 的表达明显高于DM 组和NC 组（P＜0.05）,并与胃排
空率成负相关（P＜0.05）。DM 组和NC 组差异无统计学意义（P＞0.05）。结论：PVN 区注射GLP-1 可以抑制糖尿病大鼠早期胃排
空加速，作用机制可能和促进下丘脑GLP-1 受体表达有关。 |
| 英文摘要: |
| Objective: To investigate the effection of GLP-1 injection GLP-1 in paraventricular nucleus on the expression of
GLP-1 receptor and gastric emptying in diabetic rats. Methods: Thirty Wistar rats were randomly divided into three groups: Group 1:
normal control group (NC group), Group 2: diabetic mellitus group (DM group) and Group 3: GLP-1 treated group (GLP-1 group). All of
rats were posited a cannula in PVN, the rats of Group 2 and Group 3 were induced by intraperitoneal injection of streptozotocin (STZ).
The rats of Group 3 were injected 0.5μg/0.5μl GLP-1 and isovolumic normal saline for Group 1 and Group 2 in PVN after 7-day recovery
period following surgery. Gastric emptying was measured by intragastric administration of methyl cellulose-phenol red solution. The
GLP-1 levels in the hypothalamus were measured by EISIA method, the expression levels of GLP-1R mRNA in the hypothalamus were
measured by semi-quantitative RT-PCR. Results: The gastric emptying of Group 2 showed significantly accelerated compared with that
Group 1 (P<0.05). which decreased significantly in Group 3 compared to Group 2 (P< 0.05), There was no statistical difference between
Group 2 and Group 1 (P>0.05). The expression of GLP-1 and GLP-1R mRNA in hypothalamus of Group 3 was increased significantly
compared with in the other two groups and no difference between Group 2 and Group 1 (P>0.05), which showed a negative correlation
with decrease of gastric emptying (P<0.05). Conclusion: GLP-1 Injection in PVN delays acceleration of gastric emptying during the early
stage of diabetic rats. The expression of GLP-1 receptor in the hypothalamus is promoted by GLP-1 may be the mechanism. |
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