| 常飞薛晓东△ 谢沛霖张冀北.联合应用NGF 和胰岛素对糖尿病大鼠烫伤创面表皮干细胞的影响[J].,2012,12(7):1236-1240 |
| 联合应用NGF 和胰岛素对糖尿病大鼠烫伤创面表皮干细胞的影响 |
| Effects of Nerve Growth Factor Combination with Insulinon Wound Healing with II Degree Burn and the Regenerationof Epidermis Cells in Diabetic Rats |
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| DOI： |
| 中文关键词: 糖尿病 烫伤 神经生长因子 胰岛素 表皮干细胞 |
| 英文关键词: Diabetes Burn Nerve growth factor Insulin Skin stem cells |
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| 中文摘要: |
| 目的：通过局部联合应用神经生长因子和胰岛素对糖尿病大鼠深II 度烫伤创面表皮干细胞标记物β1 整合素和角蛋白19
(K19)表达的影响，探讨神经生长因子和胰岛素联合应用于糖尿病烫伤创面治疗后对创面愈合的影响。方法：雄性wistar 大鼠腹腔
注射链脲佐菌素(STZ)建立糖尿病模型60 只，1 月后在大鼠背部造成深II 度烫伤。将大鼠随机分为糖尿病对照组(B)、胰岛素治疗
组(C)、神经生长因子治疗组(D)、神经生长因子联合胰岛素治疗组(E)，每组15 只。另取15 只正常雄性wistar 大鼠作为正常对照
组(A)。观察伤后3、7、11、15、21 d 各组创面愈合情况，检测创面β1 整合素和角蛋白19(K19)的表达并计算创面愈合率。结果：E 组
创面愈合率自第7 天起较A、B、C、D 组增加，为[(25.33±2.32)% ,(P<0.05)]；A、C、D 组创面愈合率较B 组增加分别为[(22.51±
1.78 )%，(16.68±1.95）%,(18.29±1.70)%，（P<0.05)]。E 组整合素β1 和角蛋白19(K19)表达自伤后第7 至21 天各时相点显著增
加，(P<0.05)。结论：糖尿病大鼠深II 度烫伤创面局部联合应用神经生长因子和胰岛素可促进表皮干细胞的增殖与分化从而加速
创面的愈合。 |
| 英文摘要: |
| Objective: To investigate the effects of local application of NGF combine with insulin on wound healing with II
degree deep scald and the expression of skin stem cells markers β1 integrin and keratin 19 (K19) in diabetic rats. Methods: 60 male
Wistar rats were used in the study by intraperitoneal injection of streptozotocin (STZ). Deep partial thickness scalding was engendered on
the back of the rats after one month. Then these rats were randomly divided into B (diabetes control,n=15), C (insulin treatment, n=15), D
(NGF treatment, n=15) and E (NGF and insulin treatment,n=15)groups, and group A (nomal control,n=15) was created by normal rat of
partial thickness scalding.The wound area, wound healing rate, and express of skin stem cells markers β1 integrin and keratin 19 (K19)
immunohistochemistry staining were investigated after 3, 7, 15, and 21 day post scalding days (PSDs). Results: Compared to those in
groups A、B、C and D, the wound healing rate in group E (25.33±2.32)% increased significantly since the 7th PSD (P<0.05). The wound
healing rate in group A C and D increased significantly compare with group B [(22.51±1.78)%，(16.68±1.95)%,(18.29±1.70)%，(P<0.
05)]. The expression of β1 integrin and keratin 19 (K19) in group E increased significantly compare with other groups from 7th day till
21st day(P<0.05). Conclusion: Local application of NGF combine with Insulin could be beneficial to promote skin stem cell proliferation
and differentiation in the burn wounds of the rats with diabetes and accelerate wound healing. |
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