| 刘凤娟1 张春玲2△.Egfl7 与非小细胞肺癌上皮间质转化关系的研究[J].,2012,12(6):1110-1114 |
| Egfl7 与非小细胞肺癌上皮间质转化关系的研究 |
| Relationship of Egfl7 and Epithelial-Mesenchymal Transition in Patientswith Non-small Lung Cancer |
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| DOI： |
| 中文关键词: 上皮间质转化 非小细胞肺癌 Egfl7 |
| 英文关键词: Epithelial-mesenchymal transition Non-small lung cancer Egfl7 |
| 基金项目: |
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| 中文摘要: |
| 目的：研究Egfl7 与非小细胞肺癌（NSCLC） 上皮间质转化标志物E-cadherin，Vimentin 的相关性，探讨Egfl7 是否参与
NSCLC 的上皮间质转化(EMT)。方法：分别采用免疫组化法和RT-PCR 法检测40 例NSCLC 组织和20 例肺癌旁正常肺组织中
Egfl7，E-cadherin 和Vimentin 蛋白和mRNA 的表达情况。结果：1). NSCLC 组织中的Egfl7 蛋白和mRNA 的表达水平明显高于癌
旁正常肺组织；其差异有统计学意义（P<0.05）。Egfl7 的表达水平与肺癌的临床分期、及淋巴结转移密切相关(p0.05）。结论：NSCLC 组织中Egfl7 高表达，Egfl7 可能与NSCLC 的
侵袭性相关；Egfl7 与E-cadherin 呈负相关，与Vimentin 表达成正相关，Egfl7 可能参与了NSCLC 患者的上皮间质转化(EMT)过
程，阻断Egfl7 信号可能会抑制NSCLC 患者的ENT。 |
| 英文摘要: |
| Objecuve: To study the relationship of Egfl7 and E-cadherin, Vimentin in epithelial-mesenchymal transition(EMT) and
to investigate whether of Egfl7 involved in EMT progress in the patients with non-small cell lung cancer （NSCLC）.
Methods: Forty
NSCLC and Twenty normal Pulmonary tissues were obtained Immunonistochemistry and RT-PCR were performed to determine the expressions
of Egfl7, E-cadherin and Vimentin protein and mRNA in the NSCLC tissues. To explore the correlation between Egfl7 expression
levels and EMT of NSCLC patients. Results: 1.The result of Immunohistechermial and RT-PCR analysis indicate the expression level of
Egfl7 protein and mRNA were higher in NSCLC tissues than those in normal(P<0.05). Our data also indicate that there is a highly significant
correlation of the total Egfl7 protein and mRNA expression levels with clinical degree and Lymph node metastasis (P<0.05). 2.The
expression rate of E-cadherin protein and mRNA were significantly lower in NSCLC than those in normal. The expression of E-cadherin
in metastasis group of 40 NSCLC was lower than metastases group. 3. The expression rate of Vimentin protein and mRNA were higher in
NSCLC than those in normal. The expression of Vimentin in metastasis group of 40 NSCLC was higher than metastases group. 4.The expression
of Egfl7 was negatively correlated to that of E-cadherin（r=-0.34,P<0.05）,
but positively correlated to that of Vimentin（r=0.297，
P<0.05）;
in addition, the expression of E-cadherin had no correlation with to that of Vimentin （r=0.0002，P>0.05）.
Conclusions: We
firstly demonstrated the high expression of Egfl7 in NSCLC tissues. Egfl7 may be related with aggression of NSCLC; The expression of
Egfl7 was negatively correlated to that of E-cadherin, but positively correlated to that of Vimentin. Egfl7 may be involved in EMT
progress in the patients with NSCLC, indicating that a blockage of Egfl7 may suppress EMT in NSCLC. |
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