| 张莉1 郁淼1 王浩2 严钰锋2 姚莉韵3.上海交通大学医学院附属新华医院崇明分院检验科[J].,2012,12(6):1065-1074 |
| 上海交通大学医学院附属新华医院崇明分院检验科 |
| Combining Platelet-Rich Plasma and Tissue Engineer Skin in the Treatmentof Large Skin Wound on Nude Mice |
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| DOI： |
| 中文关键词: 富血小板血浆 生长因子 皮肤创伤 |
| 英文关键词: Platelet-rich plasma( PRP) Growth factor Skin wound |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨富血小板血浆(platelet-rich plasma, PRP)结合组织工程皮肤对裸鼠巨大创面修复的影响。方法：首先，利用密度梯
度离心法制备富含生长因子的浓缩血小板的血浆，并测其所含生长因子的量；其次，在裸鼠的背侧部分构建大面积皮肤创面，分
别用人工真皮，组织工程皮肤，碱性成纤维细胞生长因子组织工程皮肤，表皮生长因子组织工程皮肤和PRP 结合组织工程皮肤修
复裸鼠巨大创面；最后，手术后不同时间间隔收集组织标本，采用HE 染色，PAS 染色和免疫组化等方法评估创面愈合情况。结果：
PRP 结合组织工程皮肤组创面修复愈合情况最好。 |
| 英文摘要: |
| Objective: To investigate the effects of tissue engineered skin in combination with PRP and other preparations on the
repair of large skin wound on nude mice. Methods: Platelet-rich plasma (PRP) from venous blood wase prepared by density gradient
centrifugation. Large skin wounds were created surgically on the dorsal part of nude mice. The wounds were then treated with either
artificial skin, tissue engineered skin, tissue engineered skin combined with basic fibroblast growth factor, and tissue engineered skin
combined with epidermal growth factor or tissue engineered skin combined with PRP. Tissue specimens were collected at different time
intervals post surgery. HE, PAS stains and immunohistochemistry were performed to assess rate of wound healing. Results: Macroscopic
observations, HE/PAS staining and immunohistochemistry revealed that the tissue engineering skin in combination with the PRP group
showed the most satisfactory wound recovery out of the five groups. |
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