| 周兰英1 孙圣华2 高健3 宋薇1 杨小仙1 唐文祥2.吲哚美辛对COPD 大鼠TNF-α 与Bcl-2 和Bax 表达的影响[J].,2012,12(6):1044-1047 |
| 吲哚美辛对COPD 大鼠TNF-α 与Bcl-2 和Bax 表达的影响 |
| The Influence of Indomethacin on the Expression of TNF-α and Bcl-2and Bax in Chronic Obstructive Pulmonary Disease Rat Model |
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| DOI： |
| 中文关键词: 慢性阻塞性肺疾病 骨骼肌 细胞凋亡 Bcl-2 Bax TNF-α |
| 英文关键词: Chronic obstructive pulmonary disease Skeletal muscle Apoptosis Bcl-2 Bax TNFα |
| 基金项目:怀化医学高等专科学校资助项目（2010KY07） |
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| 中文摘要: |
| 目的：探讨吲哚美辛对慢性阻塞性肺疾病（COPD）模型大鼠趾长伸肌TNF-α 及Bcl-2 和Bax 表达的影响。方法：100 只健康
Wistar 大鼠随机分为模型组和对照组（A 组）。模型组采用熏香烟和气管内滴注猪胰蛋白酶（PEE）法建立COPD 模型大鼠，以低于
A 组大鼠平均体重的90％判断发生营养不良的标准，将模型组大鼠分为COPD 营养正常组(B 组)和COPD 营养不良组,COPD 营
养不良组随机化原则分为COPD 营养不良生理盐水组（C 组）、吲哚美辛1 组（D 组）、吲哚美辛2 组（E 组）、吲哚美辛3 组（F 组）。
A、B、C 三组灌注等量生理盐水，D、E、F 组给予不同剂量吲哚美辛（IND）灌胃干预。TUNEL 法测定趾长伸肌细胞凋亡率，免疫组
织化学法测定Bcl-2、Bax 蛋白表达，双抗体夹心法测定血清及趾长伸肌肿瘤坏死因子α（TNF-α）浓度。结果：吲哚美辛干预后，E
组趾长伸肌细胞凋亡率、Bcl-2、Bax 的蛋白表达、趾长伸肌匀浆和吲哚美辛干预后血清TNF-α 浓度低于C、D、F 组，但高于A、B
组，P<0.05；E 组趾长伸肌Bcl-2 蛋白表达高于C、D、F 组,低于A、B 组，P<0.05。结论：Bcl-2、Bax 及TNF-α 参与COPD 模型大鼠趾
长伸肌细胞凋亡，适当剂量的吲哚美辛可改善COPD 营养不良模型骨大鼠趾长伸肌的凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of indomethacin on the expression of Bcl-2 , TNF-α and Bax in extensor digitorum
longus in the COPD rat's models. Methods: 100 healthy Wistar rats were randomly divided into model group and control group
(group A). The model group rat was performed with intratracheal instillation of porcine pancreatic elastase (PEE) and exposed to
cigarette smoke to establish COPD models. Malnutrition was defined if the body weight of the rats in the model group was lower than
90% of the mean body weight of the control group. All model rats were divided into non-malnutrition COPD group (group B) and malnutrition
COPD group, and the malnutrition COPD group rats were randomly divided into four groups: physiologic saline control group
(group C), Indomethacin intervention group 1 (group D) , Indomethacin intervention group 2 (group E) and Indomethacin intervention
group 3 (group F). Isotonic physiologic saline was administered to the group A, B and C, different dose of Indomethacin was administered
to group D, E, F by gavage. The apoptosis rates of the cell in extensor digitorum longus muscle were measured by TUNEL method,
the Bcl-2 , Bax protein expression and the concentrations of TNF-α in the serum, the extensor digitorum longus muscle from these rats
were measured by immunohistochemical method, take the method of double mono-cloned antibody for detecting the concentrations of
TNF-alpha in the serum and extensor digitorum longus muscle homogenates from these rats. Results: After indomethacin intervention,
the rates of muscle cell apoptosis, the concentration of TNF-α, Bcl-2 and the Bax protein expression, extensor digitorum longus muscle
homogenates, the concentrations of TNF-alpha in the serum in the Indomethacin intervention group 2 (group E) were lower than those in
the group C, D, F and higher than those in the group A, B (P<0.05), the Bcl-2 protein expression in extensor digitorum longus muscle in
group E was higher than that in C,D,F group and was lower than that in group A,B(P＜0.05). Conclusion: The Bcl-2 , Bax and TNF-α are
involved in occurrence of the cell apoptosis of extensor digitorum longus in COPD model rats, appropriate dose of indomethacin can improve
the apoptosis of extensor digitorum longus muscle of COPD model rats. |
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