| 刘浩郭猛章浩王洋程平纪方△.NALP1 基因与骨肉瘤细胞的相关研究[J].,2012,12(6):1040-1043 |
| NALP1 基因与骨肉瘤细胞的相关研究 |
| Research on NALP1 Gene and Osteosarcoma Cell |
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| DOI： |
| 中文关键词: 人成骨肉瘤细胞株MG-63、U-2OS NALP1 基因 细胞凋亡 |
| 英文关键词: Osteosarcoma cell line MG-63, U-2OS NALP1 gene Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨NALP1 基因在骨肉瘤细胞株MG-63、U-2OS 中的表达，以及高表达NALP1 基因对于骨肉瘤细胞体外凋亡的影
响。方法：使用RT-PCR、Western-blot 法检测骨肉瘤细胞株MG-63、U-2OS 中的mRNA 及蛋白表达水平并与人成骨细胞株
hFOB1.19 比较。将NALP1 基因转染质粒PcDNA3.1，将重组质粒转染骨肉瘤细胞，分成高表达基因组、空质粒组及对照组，加入
抗肿瘤药物顺铂及甲氨蝶呤促使肿瘤细胞凋亡，使用流式细胞仪测定各组肿瘤细胞凋亡率。结果：通过统计分析，骨肉瘤细胞株
MG-63、U-2OS 中的mRNA 及蛋白表达水平均低于人成骨细胞株hFOB1.19 （P＜0.05），NALP1 高表达组的肿瘤细胞凋亡率明显
高于空白质粒组及对照组。结论：上调骨肉瘤细胞株MG-63、U-2OS 中的NALP1 的表达量可以促进肿瘤细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the expression of NALP1 gene in osteosarcoma cell line MG-63, U-2OS and effect in vitro
apoptosis about high expression of NALP1 gene for osteosarcoma cells. Methods: We use the methods of RT-PCR, Western-blot to assay
the expression of mRNA and protein in osteosarcoma cell line MG-63, U-2OS and compare with human osteoblast cell line hFOB1.19.
We transfected the NALP1 gene into plasmid PcDNA3.1, and the recombinant plasmid was. We divided the transfected osteosarcoma
cells into three groups, highly expressed genes group, blank plasmid group and control group. Then we add the anticancer drugs cisplatin
and methotrexate to promote tumor cell apoptosis.At last we use flow cytometry in each group to determine the apoptosis of tumor cell.
Results: Through statistical analysis, we get the result that mRNA and protein expression in osteosarcoma cell line MG-63, U-2OS were
lower than the human osteoblast cell line hFOB1.19 (P <0.05), and apoptosis of highly expressed NALP1 gene group was significantly
higher than the blank plasmid group and the control group. Conclusions: Increasing the expression of NALP1 gene in osteosarcoma cell
line MG-63 and U-2OS can promote apoptosis of tumor cell. |
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