| 王阳1 沈方臻1△ 肖文静1 姚如永2 刘伟伟1.Celecoxib 联合X 线对胆管癌细胞株QBC939 凋亡的影响[J].,2012,12(6): |
| Celecoxib 联合X 线对胆管癌细胞株QBC939 凋亡的影响 |
| Effect of Celecoxib in Combination with X-ray on the Apoptosis of HumanCholangiocarcinoma Cell Line QBC939 in Vitro |
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| DOI： |
| 中文关键词: 胆管癌 塞来昔布；X 线；Survivin；凋亡 |
| 英文关键词: Cholangiocarcinoma Celecoxib X-ray Survivin Apoptosis |
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| 中文摘要: |
| 目的：研究塞来昔布联合X 线在体外环境下对人胆管癌细胞株QBC939 凋亡的影响并对其机制进行初步探讨。方法：
CCK-8 检测出不同浓度的塞来昔布及不同剂量的X 线对QBC939 细胞株的增值抑制率，确定塞来昔布组的IC50 及X 线组的
IC50。将QBC939 细胞株分为5 组：对照组（control）、X 线组（R）、塞来昔布组（C）、塞来昔布再加X 线组（C+R）,X 线再加塞来昔布
组（R+C）。用流式细胞仪检测各组的凋亡率，western blot 检测凋亡相关基因survivin 蛋白的表达。结果：联合使用塞来昔布和放疗
组的凋亡率有明显的增加，从8.268%，11.233%到15.733%，22.133% (P＜0.05)。western 结果显示联合组survivin 蛋白的表达也明
显低于对照组(P＜0.05)。先用塞来昔布再加放疗的效果要优于先用放疗后用塞来昔布的效果。结论：塞来昔布联合X 线对
QBC939 细胞株有凋亡增敏作用，作用可能机制之一是通过降低或下调凋亡相关基因survivin 蛋白的表达。 |
| 英文摘要: |
| Objective: To investigated the effect of celecoxib in combination with x-ray on the apoptosis of human cholangiocarcinoma
cell line QBC939 in vitro. Methods: The QBC939 cells were cultured in RIMP1640 supplemented with 10% FBS and treated with
celecoxib in combination with x-ray. Cell Counting Kit-8 (CCK-8) assay was used to investigate the inhibition ratio and the IC50. Cells
were divided into five groups: control group, x-ray group, celecoxib group, x-ray after the celecoxib group and x-ray before celecoxib
group. Apoptosis of each group were determined by flow cytometry. Expression of survivin was determined by western blot. Results: The
apoptosis ratio of the combination treatment groups enhanced apparently. The efficacy of the x-ray after celecoxib group was more significant
than the x-ray before celecoxib group (P＜0.05). Western blot showed that survivin expression was down-regulated obviously after
combination treatment. Conclusion: Celecoxib enhanced x-ray's effect of apoptosis-inducing. Celecoxib had the radiosensitivity-enchancing
effect on the cholangiocarcinoma QBC939 cells in vitro, one of the possible mechanisms may be that it reduced the expression or accelerating
the degradation of survivin. |
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