| 陈晨1 曹学成1△ 王志意2 赵卫东3 王昱1 陈琳1.糖尿病对大鼠骨折愈合影响的实验研究[J].,2012,12(6):1021-1024 |
| 糖尿病对大鼠骨折愈合影响的实验研究 |
| The Experimental Study on the Effects of Diabetes to Rat Fracture |
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| DOI： |
| 中文关键词: 糖尿病 骨折 IGF-1 |
| 英文关键词: Dabetes mellitus Facture Healing IGF-1 |
| 基金项目: |
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| 中文摘要: |
| 目的：观察糖尿病大鼠的骨折愈合过程，探讨糖尿病影响大鼠骨折愈合的可能的机制，为临床实践提供理论依据。方法：雄
性Wister 大鼠140 只，随机分成二组，每组70 只，A 组为糖尿病骨折组；B 组为非糖尿病骨折组。建立糖尿病动物模型后，无菌条
件下在各组大鼠胫骨中点用手术方法制成骨折模型。术后1 周、2 周、4 周、6 周、8 周各时间点进行X 线检查，观察骨折愈合情况。
术后1 周、2 周、3 周、4 周、6 周、8 周分别用ELISA 法检测血清中IGF-1 含量。分别在1、2、4、6、8 周各时间点观察5 只大鼠骨痂生
长情况并取骨折断端组织行HE 染色光镜观察。术后4 周、6 周、8 周每组处死10 只大鼠留取双侧胫骨标本，冷冻保存后集中进行
生物力学检测。结果：1、大体标本观察结果：各时间点A 组骨痂生长减缓延迟。2、X 线结果：A 组骨折愈合质量在各时间点均明显
低于B 组。3、生物力学测定结果：4 周、6 周、8 周个时间点A 组骨折处骨痂的机械强度均明显低于B 组。4、组织学染色显示：术后
各时间点1、2、4、6、8 周A 组与B 组相比骨折处局部骨痂成熟延迟并且软骨细胞肥大。5、血清IGF-1 含量测定：A 组大鼠血清中
IGF-1 含量低于B 组，且高峰延迟1 周。结论：1.患有糖尿病后大鼠骨折愈合质量差，比较容易出现愈合延迟甚至不愈合；2.患有糖
尿病的大鼠骨折后血清中的IGF-1 表达明显低于对照组，且高峰推迟1 周。 |
| 英文摘要: |
| Objective: To observe the process of fracture healing in diabetic rats . Then to investigate the possible factors related to
frature healing in diabetic rats. Methods: 140 male Wister rats were randomly divided into two groups. Group A was Diabetes group,
Group B was control group. Fracture model was made in tibial and then reducted and internal fixed. The X-rays of tibial taken in the two
groups in 1th,2th,4th,6th,8th week after the surgery to evaluation the fractue healing. After 1,2,3,4,6,8 weeks operation the serum IGF-1
was examined by ELISA assay.1,2,4,6,8 weeks after fracture, 5 rats were sacrificed in each group to observe the growth of callus, the
tissue of fracture region was taken for HE staining. At 4th, 6th and 8th week after operation biomechanical test was done. Results: Antomic
observation showed that every time point group A callus growth was slower. There were more periosteal reaction and rapid bone
reconstruction in group B than in group A. The diabetic callus mechanical strength was significantly lower than that in control group. HE
staining suggested the chondrocyte maturation hysteresis and hypertrophy in diabetic rats.Serum IGF-1 level were significantly decreased
and the peak was delayed for one week in dabetic rats. Conclusion: The diabete mellitus severely affected the rat fracture healing, and the
delayed and non-union occurace was significantly increased. The level of expression of IGF-1 in serum was decreased and peak was delayed
in diabetic rats. |
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