| 于健孙传东△ 姚如永栾坤业刘广伟张炳远.抑制人cFLIP 基因表达的shRNA 质粒的构建与功能验证[J].,2012,12(5):840-845 |
| 抑制人cFLIP 基因表达的shRNA 质粒的构建与功能验证 |
| Construction and Functional Identification of Plasmid shRNA SuppressingHuman cFLIP Expression |
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| DOI： |
| 中文关键词: cFLIP shRNA 质粒 肝癌 |
| 英文关键词: cFLIP shRNA Hepatocellular carcinoma Plasmid |
| 基金项目:山东省教育厅课题项目基金资助（J11L65） |
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| 中文摘要: |
| 目的：构建特异性抑制cFLIP 基因表达的质粒并检测其对肝癌细胞的影响。方法：根据人cFLIP mRNA 的序列，设计合成3
对cFLIP 基因的shRNA，将其连入干扰载体，转染HepG2，蛋白印迹法检测基因表达情况，以检测其对肝癌细胞的影响。结果：
构建了特异性抑制肝癌细胞中cFLIP 表达的质粒。结论：成功构建能特异且高效阻断cFLIP 表达的shRNA 表达质粒，为进一步
研究cFLIP 基因对肝癌增殖的影响及其临床应用奠定了基础。 |
| 英文摘要: |
| Objective: To construct adenoviral shRNA for inhibiting the cFLIP expression in hepatocellular carcinoma and to
investigate the influence of apoptosis on liver cancer cells (HepG2). Methods：According the sequence of the human cFLIP mRNA, four
shRNAs targeting to interference cFLIP expression were designed and combined, which were ligased into silencing vectors, were
designed. The silencing vectors were transfected into HepG2; RT-PCR and western blot were used to analyze the gene expression.
Results: The plasmid down-regulated the cFLIP expression in HepG2 was obtained. The cFLIP expression in HepG2 was significantly
suppressed by shRNA transfection. Conclusion：The shRNA plasmid down-regulated the cFLIP expression was successfully constructed,
which lay the foundation for studying cFLIP in the proliferation of human hepatocellular carcinoma and clinical application. |
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