| 朱琼1 常宇骁1 孙维娜2 杨劲3 位全芳3△.PCNA 泛素化对Hela 细胞苯并芘损伤敏感性的影响[J].,2012,12(5):815-817 |
| PCNA 泛素化对Hela 细胞苯并芘损伤敏感性的影响 |
| Effects of PCNA Ubiquitination on Benzopyrene(Bap)-InducedCytotoxicity in HeLa Cells |
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| DOI： |
| 中文关键词: PCNA 泛素化 苯并芘（BaP） 依托泊苷（VP-16） Hela 细胞 |
| 英文关键词: PCNA ubiquition Benzopyrene(BaP) Etoposide(VP-16) Hela cell |
| 基金项目:国家自然科学基金项目（No 30901220） |
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| 中文摘要: |
| 目的：观察PCNA 泛素化修饰对Hela 细胞损伤敏感性的影响。方法：Western blot 法检测His-PCNA 及His-mutant PCNA
(mPCNA，K164R)在Hela 细胞中的表达。DNA 损伤剂苯并芘（BaP）和依托泊苷（VP-16）分别处理Hela 细胞后，MTT 法检测不同
细胞系对DNA 损伤药物的敏感性；Western blot 法检测细胞PCNA 的泛素化修饰。结果：Western blot 结果显示His-PCNA 和
His-mPCNA 在Hela 细胞中稳定高表达。MTT 结果显示，苯并芘损伤后，稳定高表达mPCNA 的细胞系与野生型及高表达PCNA
细胞系相比，其细胞存活率呈明显下降趋势，而VP-16 作用后，三种细胞存活率无明显差异。Western blot 结果显示苯并芘损伤可
特异性诱导PCNA 发生泛素化修饰。结论：苯并芘损伤能够诱导PCNA 发生泛素化修饰，从而降低Hela 细胞对苯并芘损伤的敏
感性。 |
| 英文摘要: |
| Objective: To investigate the effect of ubiquitinated PCNA on BaP induced DNA damage response. Method:The
expression of the His-PCNA and His-mPCNA (mPCNA, K164R) in Hela cells were detected by Western blot. The Hela cells were dealt
by using the DNA damaging agents Benzopyrene （BaP）and Etoposide （VP-16）,
and,the sensitivity of different cell lines was detected
with MTT assay. Results: His-PCNA and His-mPCNA had stably expression in Hela cells. mPCNA-Hela cells were more sensitive to
BaP induced cytotoxicity compared with the wild type Hela cells and PCNA-Hela cells. While the survival of the three kinds of Hela cells
treated with VP-16 didn't appear significant difference. DNA Damaging agents BaP could induce PCNA ubiquitination specially.
Conclusions: PCNA ubiquitination even plays an important role in BaP induced damage response. Ubiqitinated PCNA may improve the
survival of Hela cells treated by BaP cytotoxicity . |
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