| 李广军1 姜银松2 王占青2 马玉英2△ 尹彩星2.百草枯中毒大鼠TNF-α、IL-10 的表达及大黄保护作用的研究[J].,2012,12(4):649-651 |
| 百草枯中毒大鼠TNF-α、IL-10 的表达及大黄保护作用的研究 |
| The Study on Expression of TNF-α、IL-10 in Rats with Paraquat Intoxicationand the Protection Effect of Rhubarb |
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| DOI： |
| 中文关键词: 大黄 百草枯 TNF-α IL-10 |
| 英文关键词: Rhubarb Paraquat Tumor necrosis factor-α Interleukin-10 |
| 基金项目:专利申请号：201010274655.X |
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| 中文摘要: |
| 目的：观察大黄对急性百草枯中毒大鼠TNF- α、IL-10 的干预作用，探讨其可能的作用机制。方法：90 只SD 大鼠随机分为生
理盐水对照组（A 组）、PQ(60 mg／kg)灌胃染毒组（B 组）、生大黄(300mg／kg·d)干预组（C 组），每组30 只。中毒后6h、24h、72h 分
批处死存活的大鼠，并且检测大鼠血浆TNF- α、IL-10 水平。结果：B 组、C 组TNF-α、IL-10 水平在染毒后6h 开始升高，72h 达到高
峰，与A 组相比，差异有统计学意义（P<0.05、P<0.01），在相同时间点C 组TNF- α 和IL-10 的表达低于B 组，差异均有统计学意
义（P<0.01）。B 组、C 组血浆TNF- α、IL-10 水平与中毒时间呈显著正性相关关系（r=0.849，P<0.01；r=0.790，P<0.01；r=0.0.943，P<0.
01；r=0.892，P<0.01）。结论：大黄能够通过降低百草枯中毒大鼠体内的TNF-α、IL-10 水平，减轻百草枯对大鼠的损伤作用。 |
| 英文摘要: |
| Objective: To evaluate the effect of rhubarb on tumor necrosis factor-a (TNF-α) and interleukin-10 (IL-10) in rats with
paraquat intoxication, and investigate its mechanism. Methods: 90 healthy adult SD rats were randomly divided into Group A(control
group n=30), Group B(poisoned group n=30 ) , Group C (rhubarb-treated group n=30 ) . Group B and C were treated intragastrically with
PQ at 60 mg／kg, Group C given rhubarb at 300mg／kg. d, while only saline was treated intragastrically the other two groups . Live rats
in each group were sacrificed at 6 hours,24 hours and 72 hours, for the determination of TNF-α, IL-10 in plasma of rats. Results: The
leves of TNF-α, IL-10 increased at 6 hours after paraquat intoxication in Group B and Group C, and peaked at 72 hours later, with
statistical difference from Group A （P<0.05, P<0.01）; Compared with group B, delayed，lower increasing extent，obviously reducing
tendency in group C, with statistical difference (P<0.01)．As to the leves of TNF-α, IL-10and poisoning time, there were high positive
correlations among them. Conclusions: Rhubarb ameliorates damage effect caused by PQ poisoning in rats by means of inhibiting the
expression of TNF-α, IL-10 in turn to alleviate inflammatory reaction. |
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