| 吕辉琴1 刘江伟2△ 高伟2 张东2.核因子-κB 在腹部火器伤肠管穿透后肺组织细胞凋亡中的作用*[J].,2012,12(4):642-644 |
| 核因子-κB 在腹部火器伤肠管穿透后肺组织细胞凋亡中的作用* |
| The Role of Nuclear Factor Kappa B in Pneumocyte Apoptosis Induced byIntestinal Perforations Due to Abdominal Firearm Wound* |
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| DOI： |
| 中文关键词: 核因子-κB 腹部损伤 火器伤 凋亡 肺损伤 |
| 英文关键词: NF-κB Abdominal firearm wound Pneumocyte apoptosis Lung injury |
| 基金项目:兰州军区医药卫生科研基金资助项目(LXH-2005019) |
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| 中文摘要: |
| 目的：探讨腹部火器伤肠管穿透后核因子-κB 在肺组织细胞凋亡中的作用，了解腹部火器伤肠管穿透后继发性肺损伤机
制。方法：健康长白仔猪42 头随机等分为对照组和伤后1h、2h、4h、8h、12h 和24h 组，实验组建立腹部火器伤肠管穿透模型后，用
免疫组化图像分析法测定各组肺组织内NF-κB 活性，同时用TUNEL 测定肺组织细胞凋亡变化情况。结果：伤后各组肺组织内
NF-κB 活性明显高于对照组，并于伤后8h 出现高峰。肺组织细胞凋亡指数伤后显著增高(P＜0.05)，并与NF-κB 活性变化基本
一致。结论：腹部火器伤肠管穿透后肺组织内NF-κB 活性增强，肺细胞凋亡增多，从而继发导致肺损伤。 |
| 英文摘要: |
| Objective: To investigate the effect of NF-κB in signal transduction of pneumocyte apoptosis in order to investigate
the mechanism of lung injury. Methods: 42 Chang-Bai piglets were randomized into 7 groups: control group wounded 1hour group,
2hour group, 4hour group, 8hour group, 12hour group and 24hour group. The model of intestinal perforations due to abdominal firearm
wound was established in wounded groups. Pulmonary NF-κB activity was detected by immunohistochemical staining and image
analysis in all groups. Hepatocyte apoptosis indexes were detected. Results: Levels of pulmonary NF-κB activity in wounded groups
were significantly elevated compared with that in control group, and it appeared one peaks in 8h group. Meanwhile, pneumocyte
apoptosis indexes increased markedly compared with that in control group (P<0.05). All these changes were positive correlation with the
changes of pulmonary NF-κB activity. Conclusion: After intestinal perforations duing to abdominal firearm wound, pulmonary NF-κB
activity increases and it can induce pneumocyte apoptosis and lung injury. |
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