| 王丛丛1 朱正华1 汤朝武2 石继红2 陈宇1 杨双文1 董海龙1 熊利泽1.CaMKⅡ在大鼠异氟烷全麻机制中的作用研究[J].,2012,12(3):401-403 |
| CaMKⅡ在大鼠异氟烷全麻机制中的作用研究 |
| Effect of CaMKⅡon General Anesthetics of Isoflurane in Rats |
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| DOI： |
| 中文关键词: CaMKⅡ myrAIP 异氟烷 侧脑室置管 全身麻醉 |
| 英文关键词: CaMKⅡ MyrAIP Isoflurane Intracerebroventricular cannulation General anesthetics |
| 基金项目:国家重点自然科学基金资助项目（30600582） |
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| 中文摘要: |
| 目的：探讨磷酸化钙调蛋白依赖性激酶Ⅱ (pCaMKⅡ)在异氟烷吸入全身麻醉作用中的作用及相关机制。方法：采用经侧脑
室置管全脑给药，将SD 大鼠随机分为溶剂对照组（control 组） 和CaMKⅡ抑制剂十四烷酰肉豆蔻酰- 钙调蛋白自变性抑制肽
（myrAIP）处理组(n=10)，给药40min 后行异氟烷吸入麻醉（1MAC，30min）,监测动物脑电，取脑电波平稳清晰的波段，在异氟烷麻
醉30min 时间点进行分析，并于异氟烷麻醉30min 取脑组织样本进行蛋白定量分析。结果：抑制剂组动物给药30min 时脑电抑制
波明显多于对照组（P< 0.05）；与对照组相比，抑制剂组脑组织pCaMKⅡ蛋白表达量明显降低（P< 0.05）。结论：CaMKⅡ抑制剂能
明显改变异氟烷全麻过程中大鼠的脑电变化及磷酸化蛋白表达变化，pCaMKⅡα参与了异氟烷全身麻醉的过程。 |
| 英文摘要: |
| Objective: To investigate the effectiveness and mechanism of phosphorylation of calmodulin dependent kinase Ⅱ
(pCaMKⅡ)in inhalation anesthesia of isoflurane . Methods: By cathetering into the left ventricle, SD rats were randomly divided into
control group and CaMKⅡ inhibitor myristoylated autocamtide-2-related inhibitory peptide (myrAIP)group (n=10). 40 minutes after of
administration, rats were anesthetized with isoflurane (1MAC，30min).The changes of EEG in 30min anesthetics were detected. And the
protein levels of pCaMKⅡα were detected by Western blot in 30 min of isoflurane anesthesia. Results: Inhibitive electroencepharogram
in myrAIP group was more than that in control group (P< 0.05); Protein levels of pCaMKIIα in myrAIP was lower than that in control
group (P< 0.05). Conclusion: CaMKⅡ inhibitor can significantly change the electroencepharogram and protein levels of pCaMKⅡα;
pCaMKⅡαmay be involved in the process of general anesthesia of isoflurane. |
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