| 成应向1,2 王强强1,2△ 许有泽1,2 钟振宇1,2.Bacillus spp.的筛选鉴定及浸矿渣效果研究[J].,2012,12(2):256-260 |
| Bacillus spp.的筛选鉴定及浸矿渣效果研究 |
| Study on Screening and Identification of Bacillus spp. and its LeachingProperties of Slag |
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| DOI： |
| 中文关键词: Bacillus spp. 分离鉴定 矿渣 生物浸出 |
| 英文关键词: Bacillus spp. Identification Leached residue Bio-leaching |
| 基金项目:湖南省科研机构技术创新发展专项基金支持项目（2010TF1012） |
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| 中文摘要: |
| 目的：研究Bacillus spp.的生长特性及浸镉渣效果。方法：采用9K 培养基划线分离纯化目标菌种，利用传统的测定方法测定
其生长特征，采用16S rDNA 对菌株进行分子生物学鉴定，最后对该菌株浸三种矿渣浸出效果进行了分析。结果：该微生物形态为
扁球形，革兰氏染色呈阳性，能运动，单生鞭毛；最适生长温度30℃，最适pH 值为3；代谢类型为兼性营养型；耐受NaCl 浓度为4
%；微生物氧化酶为阴性，接触酶为阳性；经鉴定该菌株属于Bacillus 属。以5%的接种量，培养基pH 为3 条件下，镉浸出效果较
好，沉渣中浸出率为90.4%，其余两种渣中浸出效果最佳。结论：Bacillus spp.可应用于镉渣中镉的浸出。 |
| 英文摘要: |
| Objective: To investigate the growth and the leaching residue of Bacillus spp. Methods: The target species were isolated
and identified by 16S rDNA electrophoresis. Traditional method was used to detect their growth characteristics. Leaching properties of
three types of slags were analyzed. Results: The spherical form of the microbial plants is flat and the gram stain G+. The organism is
movable, solitary, with an optimum growth temperature of 30 ℃, an optimum pH of 3, a facultative type of metabolism and a salinity tolerance
of 4%; The microbial oxidase is negative and catalase positive. By molecular biology analysis, it was identified that this indigenous
microorganism belongs to Bacillus spp. An optimistic result was attained under the conditions of 5 % of microbial inoculum and the
medium pH 3: the leaching rate of Cd was 90.4 % in the sediment. The optimum results can be found in the other two slags. Conclusion:
Bacillus spp. can be applied in the leaching of Cd in slag. |
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