| 袁琴1 阚卫兵1△ 宋朋飞1 张隆1 王拥军2.大鼠膝骨关节炎滑膜细胞原代培养[J].,2012,12(2):253-255 |
| 大鼠膝骨关节炎滑膜细胞原代培养 |
| Primary Culture of Rats Synoviocytes with Knee of Osteoarthritis |
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| DOI： |
| 中文关键词: 滑膜细胞 体外培养 膝骨关节炎 |
| 英文关键词: Synoviocytes Culture in vitro Knee Osteoarthritis |
| 基金项目:上海市教委课题资助（08cz041）；上海市教委高校创新团队课题资助[沪教委科(2009）6 号]； |
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| 中文摘要: |
| 目的：建立原代培养膝骨关节炎大鼠滑膜细胞的方法。方法：取膝骨关节炎大鼠膝关节滑膜组织，用酶消化法消化、分离细
胞，用DMEM 培养液进行培养并传代，观察滑膜细胞生长状况、并用细胞形态学及细胞免疫化学鉴定。结果：用酶消化法培养膝
骨关节炎滑膜细胞，方法简单、成功率高、细胞形态典型。结论：单一的胶原酶消化法是一种有效的滑膜细胞原代培养的方法，可
以满足一般实验需求。 |
| 英文摘要: |
| Objective: To develop a digestive method to obtain rats synoviocytes with knee of Osteoarthritis. Methods: Rats of OA
joint synovial layers were cut into small pieces and digested with collagenase. Cell suspension was cultured in DMEM containing 20%
fatal bovine serum. Cells were identified by morphology and immunochemistry analysis. Results: The appearance of synoviocytes is typical
and the method is simple. Conclusions: Single collagenase digestion is effective for isolation of synoviocytes, and can satisfy the general
test requirements. |
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