| 童国玉胡云沈山梅陈炜黄洪朱大龙.C2C12 细胞胰岛素增敏的机制[J].,2012,12(8):1435-1437 |
| C2C12 细胞胰岛素增敏的机制 |
| Expression of Omentin in Intestine of Mice before and after Oral GlucoseUptake and Effects of Omentin on Insulin Sensitivityin C2C12 Myotubes |
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| DOI： |
| 中文关键词: 网膜素 基因表达 C2C12 肌管细胞 胰岛素敏感性 Akt |
| 英文关键词: Omentin Gene expression C2C12 myotubes Insulin Sensitivity Akt |
| 基金项目:黑龙江省普通高等学校青年学术骨干支持计划项目（1155G33）、黑龙江省自然科学基金项目（D201103） |
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| 中文摘要: |
| 目的：观察口服葡萄糖负荷对小鼠小肠组织网膜素基因表达的影响及网膜素对C2C12 肌管细胞胰岛素敏感性的影响，并进
一步探讨其机制。方法：半定量逆转录聚合酶链反应（RT-PCR）技术检测小鼠小肠组织网膜素mRNA 的表达；葡萄糖转运实验观
察网膜素对C2C12 肌管细胞胰岛素敏感性的影响；Western blot 检测Akt (Ser473)的磷酸化水平。结果：口服葡萄糖负荷30min 后
小鼠小肠组织网膜素基因表达显著减低（P＜0.05），而60min 后恢复至负荷前水平；葡萄糖转运实验发现：网膜素作用10min 对
C2C12 肌管细胞基础葡萄糖转运无影响，但显著增加了胰岛素刺激的葡萄糖转运（P＜0.05）；Western Blot 发现：网膜素和AICAR
均显著增加了C2C12 肌管细胞Akt（Ser473）的磷酸化水平（P＜0.05）。结论：网膜素作为一种胃肠激素还受到葡萄糖负荷的调节，
在C2C12 肌管细胞，网膜素可通过增加Akt 的磷酸化发挥胰岛素增敏作用。 |
| 英文摘要: |
| Objective: To analyze the mRNA expression of omentin mRNA level in intestine of mice before and after oral glucose
uptake and to observe the effects of omentin on insulin sensitivity in C2C12 myotubes. Methods: Total RNA of intestine in mice before
and after oral glucose uptake was isolated by TRIZOL method. Semi-quantitative RT-PCR was performed to quantitate the expressions of
omentin. The uptake of 2-Deoxy- [3H]-D-glucose was used to observe glucose transport. Western blot was performed to quantitate Akt
(Ser473) phosphorylation. Results: The omentin mRNA level in intestine of mice 30min after oral glucose uptake was significantly
decreased as compared with before glucose ingestion (P<0.05). 60min after glucose intake, the omentin mRNA level recovered up to the
level before uptake. Glucose transport test revealed that C2C12 myotubes treated with omentin for 10 minutes did not influenced basal
glucose transport, but significantly increased insulin-stimulated glucose transport (P<0.05). Omentin and AICAR significently increased
Akt (Ser473) phosphorylation of C2C12 myotubes in the presence of insulin (P<0.05). Conclusion: The expression of omentin in intestine
of mice is regulated by glucose uptake. Omentin increases Akt phosphorylation in C2C12 myotubes in the presence of insulin, which may
contribute to increase insulin sensitivity. |
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