| 徐瑞芬1 张国良1 徐浩1 徐礼鲜1 冯旭阳.高氧液预处理对兔心肌缺血再灌注损伤的影响[J].,2012,12(1):23-26 |
| 高氧液预处理对兔心肌缺血再灌注损伤的影响 |
| Protective Effects of Hyperoxia Solution Preconditioning on MyocardialIschemica-Reperfusion Injury in Rabbits |
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| DOI： |
| 中文关键词: 高氧液 心肌 缺血预处理 再灌注损伤 |
| 英文关键词: Hyperoxia solution Myocardium Ischemic preconditioning Reperfusion injury |
| 基金项目:陕西省自然科学基金（2009JM4023）；陕西省社发攻关项目（2010k16-04-05）；国家自然科学基金（81000597）和（81070248） |
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| 中文摘要: |
| 目的：研究高氧液预处理对兔心肌缺血再灌注损伤的影响。方法：雄性新西兰白兔32 只，随机分为4 组（n=8）, 结扎- 开放冠
状动脉左前降支（LAD）建立心肌缺血再灌注模型。假手术组（Sham 组）只穿线环绕LAD 不结扎；吸氧组（OX 组）结扎前30 min
经鼻吸纯氧2L/ min；在结扎LAD 前30 min 分别静脉注射HO 10 ml / kg (HO1 组)、20 ml / kg (HO2 组)。于结扎LAD 前即刻（T0,基
础值）、开放LAD 前即刻（T1）、再灌注60 min（T2）及再灌注120 min（T3）时记录HR 和MAP，于T3 时抽取动脉血样3 ml, 测定血清
肌酸激酶(CK)、肌钙蛋白I (cTNI)的活性和IL-6 和TNF-α 的浓度，并测定心肌梗死范围。结果：I/S 组与T0 时比较，T 1-3 时各组
HR、MAP 进行性下降( P< 0.05 )；三组间HR、MAP 比较差异无统计学意义( P>0.05)。与Sham 组比较，I/S 组血清CK、cTNI、IL-6
和TNF-α 含量明显升高（P 0.01）；与OX 组比较，HO2 组上述酶及炎症因子浓度显著下降（P<0.01），心肌梗死范围减小(P< 0.05)。
结论：高氧液预处理可减轻兔心肌缺血再灌注损伤，机制可能与其抑制炎性反应有关。 |
| 英文摘要: |
| Objective: To invstigate the effect of hyperoxia solusion preconditioning on myocardial ischemica-reperfusion(I/R) injury
in rabbits. Methods: Thirty-two adult male rabbits weighing 2-3 kg were randomly divided into 4 groups (n=8 each): sham operation
(group S), myocardial (I/R)(group OX), group HO1, HO2 ( received hypoeroxia solusion 10,20 ml/kg respectively at 30 min before myocardial
ischemia ). Myocardial I/R was produced by temporary ligation of anterior descending branch of left coronary artery (LAD) for 30
min followed by 120 min reperfusion. Myocardial ischemia was confirmed by S-T segment elevation and change in color of myocardium.
HR and BP were monitored and recorded immediately before ligation of LAD (T0), immediately before LAD ligation was untied (T1) , 60
(T2) and 120 (T3) min after LAD was untied. Blood samples were obtained at T3 for determination of serum CK, cTNI, IL-6 and TNF-α-
concentration. Results: In group OX, HO1, HO2, HR and MAP decreased during T1-3(P< 0.05), but there was no significant difference
among the 3 groups. In I/R group, the s CK, cTNI, IL-6 and TNF-α were significantly higher than that in Sham group(P<0.01); The above
parameters in group HO2, were significantly lower and the infarct size was significantly smaller than that in group OX. Conclusion: Hyperoxia
solution preconditioning can attenuate myocardial I/R injury by inhibiting inflammatory response. |
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