| 张乾勇易龙冉莉.ω-3 多不饱和脂肪酸对肿瘤细胞Rho GTP 酶翻译后修饰的影响[J].,2012,12(1):9-11 |
| ω-3 多不饱和脂肪酸对肿瘤细胞Rho GTP 酶翻译后修饰的影响 |
| Effect of ω-3 Polyunsaturated Fatty acid on Posttranslational Modificationsof Rho GTPase in Tumor Cells |
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| DOI： |
| 中文关键词: 前列腺癌 乳腺癌 Rho 蛋白 ω-3 PUFA 翻译后修饰 |
| 英文关键词: Prostate cancer Breast cancer Rho GTPase Polyunsaturated fatty acid Posttranslational modifications |
| 基金项目:国家自然科学基金资助项目（30471465） |
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| 中文摘要: |
| 目的：观察ω-3 多不饱和脂肪酸(ω-3 Polyunsaturated fatty acid, ω-3 PUFA)对人前列腺癌PC-3 细胞和乳腺癌MDA-MB-231
细胞Rho 蛋白翻译后修饰的影响。方法：60 μmol/L 的二十碳五烯酸(eicosapentaenoic acid, EPA)和二十二碳六烯酸(docosahexaenoic
acid, DHA)处理PC-3 和MDA-MB-231 细胞24h 后，检测EPA 和DHA 对法尼基蛋白转移酶活性的影响，对Rho 蛋白的法
尼基化修饰的影响，对Rho 蛋白与GTP 结合能力的影响。结果：EPA 及DHA 均能显著下调PC-3 和MDA-MB-231 细胞法尼基蛋
白转移酶活性(P<0.01)，抑制Rho 蛋白（RhoA、Rac1、Rac2 和Cdc42）的法尼基化修饰(P<0.01)，并降低PC-3 细胞Rho 蛋白（RhoA、
Rac1 和Cdc42）与GTP 的结合能力(P<0.05)。结论：ω-3 PUFA 可能通过抑制肿瘤细胞Rho 蛋白翻译后修饰，而影响肿瘤细胞的生
物学特性。 |
| 英文摘要: |
| Objective: To investigate the effects of ω-3 Polyunsaturated fatty acid on the posttranslational modifications of Rho
GTPases in human prostate cancer cell line PC-3 and human breast cancer cell line MDA-MB-231. Methods: After a 24h pretreatment
with 60 μmol/L DHA or EPA, farnesyl protein transferase activity was assayed as the ability of cytosolic protein extracts to catalyze the
prenylation of Ras. The pretreatment cells were metabolically labeled with 1mCi/flask of [3H]farnesylpyrophosphate for 1h or with
2mCi/flask [32P]orthophosphoric acid for 4 h. Cell lysates were immunoprecipitated by incubating with RhoA,Rac1,Rac2 and Cdc42 antibody.
Transfer of [3H]farnesylpyrophosphate onto Rho GTPases was quantitated by scintillation counting. GTP- and GDP-bound Rho
GTPases were determined. Results: After treated with EPA or DHA of 60μmol/L, the farnesyl protein transferase activity in PC-3 and
MDA-MB-231 cells was deseased (P< 0.01). The posttranslational processing of Rho GTPases was inhibited by EPA and DHA (P <
0.01). A significant reduction (P< 0.05) was observed in the percentage of GTP bound RhoA,Rac1 and Cdc42 in EPA- and DHA-treated
cells compared with untreated cells. Conclusion: These results suggest that ω-3 PUFA could inhibit the posttranslational modifications of
Rho GTPases. |
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