| 魏晓晴1 吕广艳1 金海威2 崔颖1 赵莹1.DEC205在幽门螺杆菌感染胃上皮细胞中的表达[J].,2011,11(21):4033-4035 |
| DEC205在幽门螺杆菌感染胃上皮细胞中的表达 |
| Expression of DEC205 in Helicobacter pylori-Infected Human GastricEpithelial Cells |
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| DOI： |
| 中文关键词: H. pylori DEC205 胃上皮细胞 |
| 英文关键词: H. pylori-HSP60 DEC205 Gastric epithelial cells |
| 基金项目:辽宁省教育厅2009年度高等学校科研项目计划资助项目（2009A195） |
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| 中文摘要: |
| 目的：探讨幽门螺杆菌及其热休克蛋白60（H. pylori-HSP60）感染与胃上皮细胞表面DEC205 受体的关系。方法：分别用H.
pylori、H. pylori-HSP60 及E.coli LPS 刺激胃上皮细胞KATOⅢ，利用免疫荧光染色技术观察KATOⅢ细胞表面DEC205 蛋白的
表达变化，再利用RT-PCR技术，观察细胞中DEC205 mRNA对上述抗原刺激后的变化。结果：H. pylori、H. pylori-HSP60 及E.
coli LPS的刺激明显引起细胞表面DEC205 蛋白的表达以及细胞内DEC205 mRNA的产生。结论：H. pylori感染与胃上皮细胞表
面的胞吞受体DEC205 有着密切的关系。 |
| 英文摘要: |
| Objective: To investigate the relationship between Helicobacer pylori and its heat shock protein 60 (H. pylori-HSP60)
infection and human gastric epithelial cells of DEC205. Methods: After human gastric epithelial cells (KATOⅢ cells line) were infected
with H. pylori, H. pylori-HSP60 and E.coli LPS. The expression of DEC205 protein on the surface of KATOⅢ cells was observed by immunofluorescence
staining. The expression of DEC205 mRNA in KATOⅢ cells was observed by RT-PCR. Results: The expression of
DEC205 protein and mRNA were markedly increased following H. pylori, H. pylori-HSP60 and E.coli LPS stimulation in KATOⅢ cells.
Conclusion: H. pylori infection may be closely related with the expression of DEC205 in human gastric epithelial cells. |
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