易小敏张更马帅军刘克普袁建林.大鼠急性肾缺血再灌注损伤模型的建立与评估[J].,2011,11(21):4027-4029 |
大鼠急性肾缺血再灌注损伤模型的建立与评估 |
Establishment and Evaluation of Rat Acute KidneyIschemia/Reperfusion Model |
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DOI: |
中文关键词: 肾脏 缺血再灌注损伤 动物模型 |
英文关键词: Kidney Ischemia/reperfusion injury Animal model |
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中文摘要: |
目的:对现有的经腹部切口建立急性肾缺血再灌注损伤动物模型进行改良,探索建立急性肾缺血再灌注损伤模型的新方
法。方法:实验组大鼠16例,经背部切口进入腹膜后间隙,游离钳夹双侧肾动脉45 min 后开放血流,建立急性肾缺血再灌注损伤
模型;伪手术组8例,不夹闭肾动脉,余步骤与实验组相同;对照组8例无处理。术后通过建模成功率、组织病理检查、血肌酐和血
尿素氮及氧化应激水平对模型进行评估。结果:实验组15只成功建立急性肾缺血再灌注损伤模型。术后1天病理检查显示实验
组肾组织出现广泛损伤,术后实验组肾小管坏死评分、肾MDA 水平、血肌酐及血尿素氮值明显高于对照组(P<0.05)。结论:经背
部切口钳夹双侧肾动脉可建立稳定的大鼠急性肾缺血再灌注损伤模型。该造模方法简便易行,成功率高,且具备手术切口小、手
术时间短及并发症少的优点,建立的模型适合于急性肾损伤的研究。 |
英文摘要: |
Objective: To improve current acute kidney ischemia/reperfusion animal model through abdominal incision and investigate
a new approach to establish acute kidney ischemia/reperfusion animal model. Methods: Acute rat kidney ischemia/reperfusion
model was established by dorsal incision by clamping bilateral renal arteries for 45 min in experimental group (n=16); The same procedure
without renal artery clamping was implemented in sham operating group (n=8); No treatment was given to control group (n=8). Established
model was assessed by histopathological examining, concentrations of serum creatinine and blood urea nitrogen, and oxidative
stress in kidney and success ratio of acute kidney ischemia/reperfusion model establishment. Results: Acute kidney ischemia/reperfusion
model was established successfully. There were extensive injuries in experimental group, while kidney morphostructure appeared normal
in control group. Tubular injury score, MDA level, concentrations of serum creatinine and blood urea nitrogen in experimental group
after surgery were significantly higher than those in control group (P<0.05). Conclusions: Acute rat kidney ischemia/reperfusion model
can be established steadily by clamping bilateral renal arteries via dorsal incision. This procedure is simple, convenient and of high success
ratio, and have the advantages of smaller operative incision, shorter operation time and fewer complications after surgery, by which
it is suitable for in-depth research based on acute kidney injury. |
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