| 徐德华1 夏家惠2 李娟3 王丽1 杨群芳1.不同气候条件下慢性阻塞性肺疾病的病原学和免疫学研究[J].,2011,11(20):3845-3848 |
| 不同气候条件下慢性阻塞性肺疾病的病原学和免疫学研究 |
| Etiology and Immunology in Chronic Obstructive Pulmonary Disease underDifferent Climatic Conditions |
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| DOI： |
| 中文关键词: 雷暴 阴雨 台风 慢性阻塞性肺疾病 病原学 免疫 |
| 英文关键词: Thunderstorms Rainy Typhoons Chronic obstructive pulmonary disease Immune |
| 基金项目:惠州市科技局立项课题（2009Y055） |
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| 中文摘要: |
| 目的：探讨COPD 患者不同气候条件下（雷暴、阴雨、台风）的病原学和免疫功能。方法：细菌鉴定采用VITEK 全自动微生
物鉴定系统，K-B 纸片扩散法测定药敏。T 淋巴细胞亚群测定采用单克隆抗体免疫组化荧光染色法。血清免疫球蛋白(IgG, IgA,
IgM)用琼脂单向扩散法。结果：台风、雷暴、阴雨、天气气候条件60 例患者痰培养阳性率分别为45%、40%、30%，占革兰阴性菌前
2 位分别为肺炎克雷伯杆菌、流感嗜血杆菌和肺炎克雷伯杆菌、大肠埃希杆菌及流感嗜血杆菌、铜绿假单胞菌。革兰阳性菌中分别
以肺炎链球菌、金黄色葡萄球菌为主及肺炎链球菌、金黄色葡萄球菌为主和金黄色葡萄球菌、表皮葡萄球菌为主。铜绿假单胞菌、
肺炎克雷伯杆菌、流感嗜血杆菌对常用抗生素具有较高的耐药性。台风、雷暴、阴雨气候条件下3 组AECOPD 患者CD3+、CD4+、
CD4+/CD8+、IgG、IgA、IgM 均明显低于健康对照组(P<0.05),CD8+ 高于健康对照组(P<0.05),而发作期和缓解期无显著差异(P>0.05)。
三种气候条件下相互之间比较的细胞和体液免疫指标比较无显著差别(P>0.05)。结论：雷暴、阴雨、台风条件下AECOPD 的患者
存在病原学分布差异，对常用抗生素具有较高的耐药性，细胞和体液免疫功能进一步下降。但三者相互之间的免疫功能比较无明
显差异。 |
| 英文摘要: |
| Objective: To explore the etiology and immune function of COPD patients under different climatic conditions
(thunderstorms, rainy, typhoons). Methods: Bacteria were identified by VITEK automated microbial identification system. The drug
sensitivity was examined by Kribry-Bauer (K-B)paper. The T lymphocyte subgroup determination used the monoclonal antibody
immunity group fluorescence dyeing. Agar unidirectional diffusion process was applied to detect the seroimmunity globulin (IgG, IgA,
IgM). Results: The positive rates of sputum culture were 45%, 40%, 30%, respectively in Typhoons, thunderstorms, and rainy conditions.
The first two Gram-negative bacteria were Klebsiella pneumonia and Haemophilus influenza under Typhoons condition; Klebsiella
pneumonia and Escherichia coli under thunderstorms; and Haemophilus influenza and Pseudomonas aeruginosa. Gram-positive bacteria
were primarily Streptococcus pneumonia and Staphylococcus aureus in Typhoons, and Streptococcus pneumonia and Staphylococcus
aureus in thunderstorms; and Staphylococcus aureus and Staphylococcus epidermidis in rain. Pseudomonas aeruginosa, Klebsiella
pneumoniae, Haemophilus influenza had high resistance to commonly used antibiotics. Under the three weather conditions, among , the
levels of CD3+, CD4+, CD4+/CD8+ , IgG, IgA, and IgM were obviously lower in the three groups of AECOPD patients than in control
group (P<0.05); but that of CD8+ was higher than control group (P<0.05); however, compared with episode and remission, there was no
significant difference between the groups (P>0.05). The cells and humoral immune index comparison between three kinds of climate
conditions showed no statistically significant difference (P>0.05). Conclusion: Under the three kinds of climatic conditions
(thunderstorms, rainy, typhoons), AECOPD patients have difference in etiology distribution, high resistance to commonly used
antibiotics, and decline of the cellular and humoral immunity function. However, there was no statistically significant difference in cells
and humoral immune index between them. |
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