| 刘靖清1 卞红春1 胡云发3 王新卫2 陈枝楠3 陈勇3 詹爱军3.检测H5 亚型禽流感的压电免疫传感器的研究[J].,2011,11(20):3838-3840 |
| 检测H5 亚型禽流感的压电免疫传感器的研究 |
| Study on Piezoelectric Immunosensor for the Detection of H5 Sera-subtypeAvian Influenza |
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| DOI： |
| 中文关键词: 压电免疫传感器 生物自组装法 H5 亚型禽流感病毒 |
| 英文关键词: Piezoelectric immunosensor Biological self-assembly method H5 sera-type avian influenza virus |
| 基金项目:" 十一五" 国家科技支撑项目（2006BAK20A29）和广东省科技重大专项计划项目（2004A2090102）资助 |
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| 中文摘要: |
| 目的：为研制检测H5 亚型禽流感的压电免疫传感器。方法：用巯基丙酸在镀银电极石英晶体自组装巯基丙酸单分子膜再通
过N- 乙基-N′-（3- 二甲氨基）丙基碳化二亚胺盐酸（EDC)和N－羟基琥珀酰亚胺（NHS）偶联抗H5 亚型禽流感病毒的特异性单
抗构建传感器芯片，建立了可以检测H5 亚型禽流感病毒的免疫传感器。结果：结果表明，该法具有较好的特异性，不与H9 亚型
流感病毒和NDV 反应；检测灵敏度达到10-50 个EID50。结论：本文结果为检测禽流感病毒免疫传感器的进一步深入研究奠定
了基础，这为其它相关病毒的监测提供了一种新途径。 |
| 英文摘要: |
| Objective: The aim is to develop the piezoelectric immunosensor to detect H5 avian influenza (AI). Methods: Set up
the immunosensor by self-assembling Mercaptopmpionic acid(MPA) to be monolayer on the silver-coated electrode of quartz crystal and
coupling the monoclonal antibody to H5 AIV with N-ethy-N′-(3-dimethyl aminopropyl）carbodiimide hydrochloride(EDC) and N-hydr
oxysuccinimide ( NHS）,the chip of immunosensor to detect H5 AIV was established. Results: The results showed that the immunosensor
displayed better specificity to H5 AIV and no respond to H9AIV and NDV when being used for detection. The sensitivity test indicated
the detecting limit for the H5 AIV could reach to 10-50 EID50. Conclusion: The method provided a foundation for further research on
immunosensor for detecting AIV and it could be a new exploration to detect other viruses with the same technique in animal husbandry. |
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