| 梁伟徐兰杨荣秉初明连觅朱蕴兰余培峰徐碧荷王月丹.DNA 甲基转移酶基因干扰对K562 细胞癌- 睾丸抗原表达的影响[J].,2011,11(20):3801-3804 |
| DNA 甲基转移酶基因干扰对K562 细胞癌- 睾丸抗原表达的影响 |
| Effects of Interference of DNA Methyltransferases in Expression ofCancer/Testis Antigens in K562 Cell |
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| DOI： |
| 中文关键词: 甲基转移酶 癌- 睾丸抗原 肿瘤 K562 细胞 |
| 英文关键词: Methyltransferases Cancer-testis antigen Tumor K562 cell line |
| 基金项目:国家自然科学基金资助项目（30771995） |
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| 中文摘要: |
| 目的：探讨抑制甲基转移酶(DNMT) 对K562 细胞中癌- 睾丸抗原表达的影响及其机制。方法：分别采用针对DNMT 家族不
同成员的siRNA 转染K562 细胞,，采用RT-PCR 检测细胞中DNMT 及癌- 睾丸抗原的水平表达，并采用甲基化特异PCR(MSP)
检测部分癌- 睾丸抗原基因启动子的甲基化状态。结果：经siRNA 干扰后，K562 细胞中DNMT1、DNMT3a 和DNMT3b 的表达量
均明显降低，癌- 睾丸抗原CT10 的启动子区序列发生了去甲基化，但处于非甲基化状态的MAGE-A1 启动子区没有发生任何改
变。干扰DNMT 组的K562 细胞，再表达癌- 睾丸抗原CT10、PRAME 和CT9，而MAGE-A1、SSX-1 的表达上调，但是NY-ESO-1、
HCA587 和HCA661 的表达状况均没有任何影响。结论：在K562 细胞中,干扰DNMT 可使部分癌- 睾丸抗原基因的启动子区发
生去甲基化，从而导致相应的癌- 睾丸抗原分子的再表达或表达增加。 |
| 英文摘要: |
| Objective: To investigate the effects and the underlying mechanisms of inhibiting three kinds of DNA methyltransferases
(DNMT1, DNMT3a and DNMT3b) on the expression of cancer/ testis antigen (CTA) in K562 cell. Methods: Transient
transfection of K562 cell with siRNA was targeted against different members of DNMT family. RT-PCR was used to detect the
expressions of DNMTs and CTAsm, and the promoter methylation of partial CTA genes was detected with methylation specific PCR
(MSP). Results: The expressions of DNMT1, DNMT3a and DNMT3b declined significantly after siRNA interference in K562 cells.
Demethylation occurred in the promoter region of CT10, while the promoter region of MAGE-A1 was in a state of unmethylation. CT10,
PRAME and CT9, re-expressed in all transfected cells group; The expressions of MAGE-A1and ssx-1 were up-regulated in transfected
cells group; There was not expression of NY-ESO-1 HCA587 and HCA661 in all cells. Conclusions: In K562 cells, DNMT can
up-regulate the expression of CTA by demethylated the promoter. |
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