陈远崇1 陈竹雨2 侯建华1 闫山英1 席锋祥1.MBL 在结肠直肠癌中的基因分型[J].,2011,11(17):3306-3310 |
MBL 在结肠直肠癌中的基因分型 |
MBL Genotypes in Colorectal Cancer |
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DOI: |
中文关键词: 结肠直肠癌 甘露糖结合凝集素 基因型 单核苷酸多态性 |
英文关键词: Colorectal cancer Mannan-binding lectin(MBL) Gene type Single-nucleotide polymorphisms (SNPs) |
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中文摘要: |
目的:MBL 是补体激活凝集素途径的关键因素。MBL 基因多态性影响MBL 血清水平。结肠直肠癌患者血清MBL 水平升
高,低水平的MBL 则预示着术后肺炎的发生,目前还不清楚此相关性是否与遗传相关。本实验分析调查了结肠直肠癌患者和健
康对照者的MBL 基因分型,评估基因分析和复发率、生存率之间潜在的相关性。方法:使用TaqMan 基因分型分析法和实时定量
PCR 分析MBL 基因的4 个SNP、启动子区2 个SNP、非编码区1 个SNP;ELISA 测定标本血清MBL 含量。结果:所有标本中发
现了8 种不同的MBL 单体型,它们在患者和健康者中出现频率几乎是完全一样的;YA/YA 基因型与高水平的MBL 相关,
YO/YO 与低水平的MBL 水平相关,6 种不同基因型CRC 患者的MBL 水平存在着明显不同。结论:MBL 基因型与血清MBL 浓
度显著相关(P<0.0001);突变型B,C,D 和启动子单体型Y,X 对MBL 含量的影响起主要作用;MBL 基因型和术后感染并发症没
有明显相关性(P=0.33),与复发癌和存活时间也没有明显相关性(P=0.74)。因此,从基因水平还不能解释为何结肠直肠癌患者血
清MBL 水平增加。对比已经检测出的血清MBL 水平,其基因型还不能预测结肠直肠癌患者的疾病进程。 |
英文摘要: |
Objective: Mannan-binding lectin (MBL) is key factors of the lectin pathway of complement activation. Polymorphisms
of the MBL-2 genes affect serum levels of MBL. In patients with colorectal cancer (CRC), the MBL serum levels are increased
and low MBL levels predict post-operative pneumonia. It is not known whether these associations are genetically based. In this study, the
MBL genotypes are investigated in patients with CRC and healthy controls. The potential association between genetic profile and recurrence
and survival is evaluated. Methods: Four single-nucleotide polymorphisms (SNPs) of MBL, two SNPs of promoter region and one
SNP of untranslated region were analysed using TaqMan assays. The concentration of MBL in the sera were detected by ELISA. Results:
Eight different MBL haplotypes were found among the CRC patients and healthy individuals, The frequency of each haplotype in the
patients was not different from that in healthy controls, YA?YA genotype was associated with the highest MBL levels, and Y0/Y0 and
XA/Y0 with the lowest levels. a significant difference between the serum levels of MBL in the six genotypes. Conclusion: MBL genotype
significantly associated with MBL concentration in serum (P<0.0001). Variants B, C and D and the promoter haplotypes Y and X have
dominant effects on the concentration of MBL. No significant association between MBL genotype and post-operative infectious complications
(P=0.33), recurrent cancer or survival (P=0.74) was found. Thus, the increased serum levels of MBL found in patients with CRC
are not explained for by genetic profiles. In contrast to what has been demonstrated for serum levels of MBL, the genotypes do not predict
disease course of the CRC patients. |
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