| 庄玉玉严沁姜飞洲王清莹周珑万小平.GRP94 和CD8 在宫颈病变组织中的表达及意义[J].,2011,11(17):3233-3239 |
| GRP94 和CD8 在宫颈病变组织中的表达及意义 |
| Expression and Significance of GRP94 and CD8 in the PathologicalTissues of Cervix |
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| DOI： |
| 中文关键词: 子宫颈肿瘤 GRP94 CD8 免疫组织化学 Western blot |
| 英文关键词: Uterine cervical neoplasms GRP94 CD8 Immunohistochemistry Western blot |
| 基金项目:上海市科委基础研究重点项目（10JC1415600） |
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| 中文摘要: |
| 目的：探讨葡萄糖调节蛋白94（glucose-regulated protein94，GRP94）和CD8 在宫颈病变组织中的表达及与HPV 感染的关
系。方法：采用免疫组化S-P 法检测32 例原发宫颈癌、27 例宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）及40 例慢性宫
颈炎组织中GRP94 和CD8 的表达及定位；采用western blot 技术检测6 例宫颈癌及6 例正常宫颈组织中GRP94 和CD8 的表
达。结果：①在宫颈癌、CIN2/3、CIN1 及慢性宫颈炎组织中，GRP94 的阳性表达率分别为87.5 %、82.4 %、40 %和22.5 %；CD8 的阳
性表达率分别为28.1 %、64.7 %、90 %和97.5 %；GRP94 在宫颈癌和CIN2/3 中的表达均显著高于慢性宫颈炎和CIN 1 组织（P 均
<0.05）；CD8 在宫颈癌组的表达显著低于慢性宫颈炎组、CIN1 组及CIN2/3 组（P 均<0.05）。②Western blot 结果示GRP94 在宫颈
癌组织中的表达显著高于正常宫颈组织（P<0.01）；CD8 在宫颈癌组织中的表达显著低于正常宫颈组织（P<0.01）。③GRP94 的表
达与宫颈癌分化程度、有无脉管侵袭有关（P<0.05），而与年龄、病理类型、临床分期及有无淋巴结转移无关（P>0.05）；CD8 的表达
与有无脉管侵袭有关（P<0.05），而与年龄、病理类型、临床分期、分化程度及有无淋巴结转移无关（P>0.05）。④宫颈病变组织中，
GRP94 的表达与HPV 感染呈正相关（rs =0.377，P=0.000）；CD8 的表达与HPV 感染呈负相关（rs = -0.395，P=0.000）；GRP94 与
CD8 的表达呈负相关（rs = -0.608，P=0.000）。结论：GRP94 表达可能是宫颈CIN 进展及宫颈癌预后判断的重要预测指标。 |
| 英文摘要: |
| Objective: To investigate the expression of glucose-regulated protein 94 (GRP94) and CD8 in the pathological tissues
of cervix and their relationship with HPV infection. Methods: S-P immunohistochemical staining was used to detect the expression and
localization of GRP94 and CD8 in cervical tissues, which contained 32 cases of cervical cancer, 27 cases of cervical intraepithelial neoplasia
(CIN) and 40 cases of chronic cervicitis ; Western blot was used to detect the exprssion of GRP94 and CD8 in 6 cervical cancer
and 6 normal cervical tissues. Results: ① In cervical cancer, CIN2/3, CIN1 and chronic cervicitis tissue the positite expression rates of
GRP94 were 87.5%, 82.4%, 40% and 22.5%, respectively, while the positite expression rates of CD8 were 28.1%, 64.7%, 90% and
97.5%, respectively; The expression of GRP94 in cervical cancer and CIN2/3 were significantly higher than that in chronic cervicitis and
CIN1 (P<0.05); The expression of CD8 in cervical cancer was significantly lower than that in chronic cervicitis, CIN1 and CIN2/3 (P<0.
05). ② The expression level of GRP94 in cervical cancer was significantly higher than that in normal cervical tissue (P<0.01); The expression
of CD8 in cervical cancer was significantly lower than that in normal cervical tissue (P<0.01). ③ GRP94 protein expression had
close relationship with differentiation and vessel invasion (P<0.05), but no relationship with age, clinical stage, histological type and
lymph node metastasis (P>0.05). CD8 protein expression had close relationship with vessel invasion (P<0.05), but no relationship with
age, clinical stage, histological type, differentiation and lymph node metastasis ( P>0.05). ④ In cervical tissue, GRP94 expression was
positively correlated with HPV infection (rs =0.377, P=0.000); CD8 expression was negatively correlated with HPV infection (rs =-0.395,
P=0.000); GRP94 was negatively correlated with CD8 (rs =-0.608, P=0.000). Conclusion: GRP94 may serve as potential predicters for
progression of CIN and prognosis of cervical cancer. |
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