| 徐新颖于竹芹李震李晓丹郭云良△.昆布（海带）对高脂血症大鼠血脂的调节作用和机制[J].,2011,11(14):2642-2646 |
| 昆布（海带）对高脂血症大鼠血脂的调节作用和机制 |
| Regulating Effect of Laminaria Japonica Powder on Serum Lipids ofHyperlipidemia in Rats |
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| DOI： |
| 中文关键词: 昆布（海带） 高脂血症 超氧化物歧化酶 谷胱甘肽过氧化物酶 大鼠 |
| 英文关键词: Laminaria japonica Hyperlipidemia SOD GSH-PX Rats |
| 基金项目:国家自然科学基金项目（40976085）；青岛大学硕士优秀论文培育项目（YSPY2010010） |
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| 中文摘要: |
| 目的：探讨昆布（海带）在实验性高脂血症大鼠中的降血脂作用和机制。方法：健康雌性Wistar 大鼠40 只，应用高脂饲料喂
养方法建立高脂血症动物模型，海带粉饲料喂养干预治疗。生化法检测大鼠血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白
（LDL）和高密度脂蛋白（HDL）水平。硫代巴比妥酸法和硝酸还原酶法分别检测脂质过氧化物丙二醛（MDA）和一氧化氮（NO）含
量，黄嘌呤氧化酶法和化学比色法分别测定超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性。结果：经海带干预治
疗后，动物血清TG、TC 和LDL 水平较模型组显著降低、HDL 水平显著升高（P<0.05）。治疗组动物血清和肝组织MDA 和NO 水
平显著低于、而SOD 和GSH-PX 活性均显著高于模型组（P<0.05）。结论：海带可能影响TG、TC、LDL 和HDL 等组分的代谢，通过
增强抗氧化SOD 和GSH-PX 的活性，降低体内MDA 和NO 的水平，发挥调节血脂水平的作用。 |
| 英文摘要: |
| Objective: To investigate the effect and mechanism of Laminaria japonica (Laminariales, Phaeophyta) on serum lipid
of hyperlipidemia in rats. Methods: Forty healthy female Wistar rats were used to establish hyperlipidemia models by feeding fat-rich forage
and the kelp powder was applied as raw materials for potential marine drugs. The levels of serum lipid including the triglyceride
(TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL) were detected by biochemical assay. The
concentrations of lipid perioxide malondialdehyde (MDA) and nitric oxide (NO) were respectively measured by thiobarbituric acid assay
and nitrate reductase assay. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were respectively determined
by xanthinoxidase assay and chemical colormetry. Results: After treated with kelp feeds, the serum levels of TG, TC and LDL decreased
while HDL increased significantly than those in model group rats (P<0.05). After treatment with kelp, the levels of MDA and NO
in serum and hepatic tissue were lower than those in the model group rats (P<0.05), while the activities of SOD and GSH-PX were significantly
higher than those in the model group rats (P<0.05). Conclusion: L.japonica may interference the metabolism of TG, TC, LDL and
HDL and by increasing the activities of SOD and GSH-PX to reduce the levels of MDA and NO to regulate the levels of serum lipids. |
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